Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RR01091
Host Cell : TE-1 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RR01091 |
| Description | IL6pro-Luc/EF1α-mCherry Reporter Cell Line - TE-1 |
| Product Type | Signaling pathway reporter cell line |
| Target Gene | IL6pro-Luc/EF1α-mCherry |
| Host Cell | TE-1 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Signaling pathway studies and screening assay |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent cell line |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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We purchased this cell line for our esophageal cancer inflammation studies, and the performance has been outstanding. The dual-reporter system is incredibly convenient; having the EF1α-mCherry constitutive expression allows us to easily normalize for cell number differences across wells, which significantly improved our data accuracy. The luciferase response to induction was robust and reproducible. Highly recommended for anyone studying IL-6 pathway regulation.
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