Human XYLT2 KO Cell Line-Hela

Name: Human XYLT2 KO Cell Line-Hela
SKU: CSC-RT2840
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2840

Target Gene : XYLT2 Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2840
Description	This cell is a stable cell line with a homozygous knockout of human XYLT2 using CRISPR/Cas9.
Target Gene	XYLT2
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	XYLT2 xylosyltransferase II [ Homo sapiens ]
Gene Symbol	XYLT2
Synonyms	XT2; XT-II; PXYLT2; xylT-II
Gene Description	xylosyltransferase II
GeneID	64132
Uni ProtID	Q9H1B5
mRNA Refseq	NM_022167.2
Protein Refseq	NP_071450.2
Chromosome Location	17q21.33
Function	acetylglucosaminyltransferase activity; protein xylosyltransferase activity;
Pathway	Glycosaminoglycan biosynthesis - chondroitin sulfate, organism-specific biosystem; Glycosaminoglycan biosynthesis - chondroitin sulfate, conserved biosystem; Glycosaminoglycan biosynthesis - heparan sulfate, organism-specific biosystem; Glycosaminoglycan biosynthesis - heparan sulfate, conserved biosystem; Glycosaminoglycan biosynthesis, linkage tetrasaccharide, organism-specific biosystem; Glycosaminoglycan biosynthesis, linkage tetrasaccharide, conserved biosystem; chondroitin sulfate biosynthesis, organism-specific biosystem;
MIM	608125

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