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Human TGFBR2/SMAD234-Luc Reporter Cell Line - HEK293

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RR01252

Host Cell :   HEK293 Size :   >1x106 frozen cells/vial

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Cat. No. CSC-RR01252
Description HEK293-Human-TGFBR2/SMAD234-Luc cell line is engineered to stably express human TGFBR2 and a luciferase reporter system under the control of SMAD2/3/4 response element. The overexpression of human TGFBR2 has been validated by QPCR analysis, and the functional activity of this cell line has been verified through TGFB1 stimulation treatment.
Product Type Signaling Pathway Reporter Cell Lines
Target Gene TGFBR2/SMAD234-Luc
Gene Species Human
Host Cell HEK293
Host Cell Species Homo sapiens (Human)
Applications Employed to monitor TGF-β signaling activation through TGFBR2 (TGF-β receptor 2) and SMAD2/3/4-responsive elements; useful for studying TGF-β-mediated cell processes or screening TGF-β pathway modulators.
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent cell line
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
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Outstanding Performance

We purchased this cell line to screen for novel TGFBR2 inhibitors, and the results have been impressive. The signal-to-noise ratio is outstanding, providing a robust readout for SMAD2/3/4 signaling.

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