Human SREBF1 KO Cell Line-Hela

Name: Human SREBF1 KO Cell Line-Hela
SKU: CSC-RT2843
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2843

Target Gene : SREBF1 Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2843
Description	This cell is a stable cell line with a homozygous knockout of human SREBF1 using CRISPR/Cas9.
Target Gene	SREBF1
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SREBF1 sterol regulatory element binding transcription factor 1 [ Homo sapiens ]
Gene Symbol	SREBF1
Synonyms	SREBF1; sterol regulatory element binding transcription factor 1; sterol regulatory element-binding protein 1; bHLHd1; SREBP 1c; SREBP1; SREBP-1; class D basic helix-loop-helix protein 1; SREBP-1c;
GeneID	6720
Uni ProtID	P36956
mRNA Refseq	BC057388
Chromosome Location	17p11.2
Function	DNA binding; protein binding; sequence-specific DNA binding transcription factor activity; sterol response element binding;
Pathway	Adipogenesis, organism-specific biosystem; Caspase cascade in apoptosis, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Id Signaling Pathway, organism-specific biosystem; Insulin signaling pathway, organism-specific biosystem;
MIM	184756

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