Human SHMT1 KO Cell Line-HEK293T

Name: Human SHMT1 KO Cell Line-HEK293T
SKU: CSC-RT2837
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2837

Target Gene : SHMT1 Host Cell : HEK293T

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2837
Description	This cell is a stable cell line with a homozygous knockout of human SHMT1 using CRISPR/Cas9.
Target Gene	SHMT1
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SHMT1 serine hydroxymethyltransferase 1 (soluble) [ Homo sapiens ]
Gene Symbol	SHMT1
Synonyms	SHMT; CSHMT
Gene Description	serine hydroxymethyltransferase 1 (soluble)
GeneID	6470
Uni ProtID	P34896
mRNA Refseq	NM_148918.1
Protein Refseq	NP_683718.1
Chromosome Location	17p11.2
Function	L-allo-threonine aldolase activity; amino acid binding; glycine hydroxymethyltransferase activity; glycine hydroxymethyltransferase activity; protein homodimerization activity; pyridoxal phosphate binding;
Pathway	C1-unit interconversion, eukaryotes, organism-specific biosystem; C1-unit interconversion, eukaryotes, conserved biosystem; Carnitine synthesis, organism-specific biosystem; Cyanoamino acid metabolism, organism-specific biosystem; Cyanoamino acid metabolism, conserved biosystem; Glycine, serine and threonine metabolism, organism-specific biosystem; Glycine, serine and threonine metabolism, conserved biosystem;
MIM	182144

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