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Human PYGB KO Cell Line-HeLa

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT2992

Target Gene :   PYGB Host Cell :   HeLa

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT2992
Description This cell is a stable cell line with a homozygous knockout of human PYGB using CRISPR/Cas9
Target Gene PYGB
Host Cell HeLa
Host Cell Species Homo sapiens (Human)
Size 1 vial (>10^6 cell/vial)
Validation Sequencing
Storage Liquid Nitrogen
Shipping Dry ice package
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name PYGB phosphorylase, glycogen; brain [ Homo sapiens ]
Gene Symbol PYGB
Synonyms GPBB
Gene Description phosphorylase, glycogen; brain
GeneID 5834
Uni ProtID P11216
mRNA Refseq NM_002862.3
Protein Refseq NP_002853.2
Chromosome Location 20p11.2-p11.1
Function drug binding; glycogen phosphorylase activity; protein homodimerization activity; pyridoxal phosphate binding;
Pathway Glucose metabolism, organism-specific biosystem; Glycogen Metabolism, organism-specific biosystem; Glycogen breakdown (glycogenolysis), organism-specific biosystem; Insulin signaling pathway, organism-specific biosystem; Insulin signaling pathway, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of carbohydrates, organism-specific biosystem;
MIM 138550
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