Human PRKAR1B KO Cell Line-HeLa

Name: Human PRKAR1B KO Cell Line-HeLa
SKU: CSC-RT2974
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2974

Target Gene : PRKAR1B Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2974
Description	This cell is a stable cell line with a homozygous knockout of human PRKAR1B using CRISPR/Cas9
Target Gene	PRKAR1B
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PRKAR1B protein kinase, cAMP-dependent, regulatory, type I, beta [ Homo sapiens ]
Gene Symbol	PRKAR1B
Synonyms	PRKAR1
GeneID	5575
Uni ProtID	P31321
mRNA Refseq	NM_001164758.1
Protein Refseq	NP_001158230.1
Chromosome Location	7p22
Function	cAMP binding; cAMP-dependent protein kinase regulator activity;
Pathway	Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Aquaporin-mediated transport, organism-specific biosystem; Ca-dependent events, organism-specific biosystem; CaM pathway, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Calmodulin induced events, organism-specific biosystem;
MIM	176911

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