Human PI4KB KO Cell Line-HeLa

Name: Human PI4KB KO Cell Line-HeLa
SKU: CSC-RT2968
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2968

Target Gene : PI4KB Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2968
Description	This cell is a stable cell line with a homozygous knockout of human PI4KB using CRISPR/Cas9
Target Gene	PI4KB
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PI4KB phosphatidylinositol 4-kinase, catalytic, beta [ Homo sapiens ]
Gene Symbol	PI4KB
Synonyms	PI4KB; phosphatidylinositol 4-kinase, catalytic, beta; PIK4CB; phosphatidylinositol 4-kinase beta; PI4K BETA; pi4K92; PtdIns 4-kinase beta; type III phosphatidylinositol 4-kinase beta; phosphatidylinositol 4-kinase, wortmannin-sensitive; NPIK; PI4K92; PI4KBETA; PI4K-BETA; PI4KIIIBETA; FLJ30129; DKFZp686O1820;
Gene ID	5298
Uni Prot ID	Q9UBF8
m RNA Refseq	BC000029
Chromosome Location	1q21
Function	1-phosphatidylinositol 4-kinase activity; ATP binding; kinase activity; nucleotide binding;
Pathway	3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; D-myo-inositol -trisphosphate biosynthesis, organism-specific biosystem; D-myo-inositol -trisphosphate biosynthesis, conserved biosystem; Inositol phosphate metabolism, organism-specific biosystem; Inositol phosphate metabolism, conserved biosystem; Inositol phosphate metabolism, PI=>
MIM	602758

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