Human PDE8A KO Cell Line-Hela

Name: Human PDE8A KO Cell Line-Hela
SKU: CSC-RT2836
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2836

Target Gene : PDE8A Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2836
Description	This cell is a stable cell line with a homozygous knockout of human PDE8A using CRISPR/Cas9.
Target Gene	PDE8A
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PDE8A phosphodiesterase 8A [ Homo sapiens ]
Gene Symbol	PDE8A
Synonyms	HsT19550
Gene Description	phosphodiesterase 8A
GeneID	5151
Uni ProtID	O60658
mRNA Refseq	NM_173454.1
Protein Refseq	NP_775656.1
Chromosome Location	15q25.3
Function	3,5-cyclic-AMP phosphodiesterase activity; 3,5-cyclic-nucleotide phosphodiesterase activity; metal ion binding; two-component response regulator activity;
Pathway	G Protein Signaling Pathways, organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Morphine addiction, organism-specific biosystem; Morphine addiction, conserved biosystem; Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem;
MIM	602972

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