Human MTDH KO Cell Line-HeLa

Name: Human MTDH KO Cell Line-HeLa
SKU: CSC-RT2989
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2989

Target Gene : MTDH Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2989
Description	This cell is a stable cell line with a homozygous knockout of human MTDH using CRISPR/Cas9
Target Gene	MTDH
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	MTDH metadherin [ Homo sapiens ]
Gene Symbol	MTDH
Synonyms	3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3
Gene Description	metadherin
GeneID	92140
Uni ProtID	Q86UE4
mRNA Refseq	NM_178812.3
Protein Refseq	NP_848927.2
Chromosome Location	8q22.1
Function	NF-kappaB binding; RNA polymerase II transcription factor binding; protein binding; transcription coactivator activity;
Pathway	Validated targets of C-MYC transcriptional activation, organism-specific biosystem;
MIM	610323

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