Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT2913
Target Gene : LYPLA1 Host Cell : HEK293T
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT2913 |
| Description | This cell is a stable cell line with a homozygous knockout of human LYPLA1 using CRISPR/Cas9 |
| Target Gene | LYPLA1 |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Size | 1 vial (>10^6 cell/vial) |
| Validation | Sequencing |
| Storage | Liquid Nitrogen |
| Shipping | Dry ice package |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | LYPLA1 lysophospholipase I [ Homo sapiens ] |
| Gene Symbol | LYPLA1 |
| Synonyms | LPL1; APT-1; hAPT1; LYSOPLA |
| Gene Description | lysophospholipase I |
| GeneID | 10434 |
| Uni ProtID | O75608 |
| mRNA Refseq | NM_006330.2 |
| Protein Refseq | NP_006321.1 |
| Chromosome Location | 8q11.23 |
| Function | lysophospholipase activity; palmitoyl-(protein) hydrolase activity; palmitoyl-(protein) hydrolase activity; |
| Pathway | Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of nitric oxide, organism-specific biosystem; eNOS activation, organism-specific biosystem; eNOS activation and regulation, organism-specific biosystem; |
| MIM | 605599 |
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