Human JUNB KO Cell Line-HeLa

Name: Human JUNB KO Cell Line-HeLa
SKU: CSC-RT2921
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2921

Target Gene : JUNB Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2921
Description	This cell is a stable cell line with a homozygous knockout of human JUNB using CRISPR/Cas9
Target Gene	JUNB
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	JUNB jun B proto-oncogene [ Homo sapiens ]
Gene Symbol	JUNB
Synonyms	AP-1
Gene Description	jun B proto-oncogene
Gene ID	3726
Uni Prot ID	P17275
m RNA Refseq	NM_002229.2
Protein Refseq	NP_002220.1
Chromosome Location	19p13.2
Function	DNA binding; double-stranded DNA binding; protein binding; protein dimerization activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; transcription coactivator activity; transcription corepressor activity;
Pathway	ATF-2 transcription factor network, organism-specific biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Calcium signaling in the CD4+ TCR pathway, organism-specific biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; IL6-mediated signaling events, organism-specific biosystem;
MIM	165161

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