Human GLB1 KO Cell Line-HeLa

Name: Human GLB1 KO Cell Line-HeLa
SKU: CSC-RT2972
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2972

Target Gene : GLB1 Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2972
Description	This cell is a stable cell line with a homozygous knockout of human GLB1 using CRISPR/Cas9
Target Gene	GLB1
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	GLB1 galactosidase, beta 1 [ Homo sapiens ]
Gene Symbol	GLB1
Synonyms	EBP; ELNR1; MPS4B
Gene Description	galactosidase, beta 1
Gene ID	2720
Uni Prot ID	P16278
m RNA Refseq	NM_000404.2
Protein Refseq	NP_000395.2
Chromosome Location	3p21.33
Function	beta-galactosidase activity; cation binding; galactoside binding; protein binding;
Pathway	Disease, organism-specific biosystem; Galactose metabolism, organism-specific biosystem; Galactose metabolism, conserved biosystem; Glycosaminoglycan degradation, organism-specific biosystem; Glycosaminoglycan degradation, conserved biosystem; Glycosaminoglycan metabolism, organism-specific biosystem; Glycosphingolipid biosynthesis - ganglio series, organism-specific biosystem;
MIM	611458

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