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Human FGFR2/SRE-Luc Reporter Cell Line - HEK293

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RR01254

Host Cell :   HEK293 Size :   >1x106 frozen cells/vial

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Cell Culture Information

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Cat. No. CSC-RR01254
Description HEK293-Human-FGFR2/SRE-Luc cell line is engineered to stably express human FGFR2 and a luciferase reporter system under the control of SRE. The overexpression of human FGFR2 has been validated by QPCR analysis, and the functional activity of this cell line has been verified through FGF1 stimulation treatment.
Product Type Signaling Pathway Reporter Cell Lines
Target Gene FGFR2/SRE-Luc
Gene Species Human
Host Cell HEK293
Host Cell Species Homo sapiens (Human)
Applications Applied to measure FGFR2 (fibroblast growth factor receptor 2)-mediated signaling via SRE (serum response element); suitable for studying FGF-induced cell proliferation/differentiation or screening FGFR2-targeted drugs.
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent cell line
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
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Streamlined Our FGFR2 Screening Workflow

We needed a stable system to monitor FGFR2 activity, and this product delivered. The SRE-Luciferase response is immediate and dose-dependent. The cell line arrived healthy and established quickly in our culture system. Highly recommended for kinase research.

Germany

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