Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RR01254
Host Cell : HEK293 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RR01254 |
| Description | HEK293-Human-FGFR2/SRE-Luc cell line is engineered to stably express human FGFR2 and a luciferase reporter system under the control of SRE. The overexpression of human FGFR2 has been validated by QPCR analysis, and the functional activity of this cell line has been verified through FGF1 stimulation treatment. |
| Product Type | Signaling Pathway Reporter Cell Lines |
| Target Gene | FGFR2/SRE-Luc |
| Gene Species | Human |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Applied to measure FGFR2 (fibroblast growth factor receptor 2)-mediated signaling via SRE (serum response element); suitable for studying FGF-induced cell proliferation/differentiation or screening FGFR2-targeted drugs. |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent cell line |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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We needed a stable system to monitor FGFR2 activity, and this product delivered. The SRE-Luciferase response is immediate and dose-dependent. The cell line arrived healthy and established quickly in our culture system. Highly recommended for kinase research.
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