Human FANCM Knockout Cell Line-HCT116

Name: Human FANCM Knockout Cell Line-HCT116
SKU: CSC-RT2827
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2827

Target Gene : FANCM Host Cell : HCT116

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2827
Description	This cell is a stable cell line with a homozygous knockout of human FANCM using CRISPR/Cas9.
Target Gene	FANCM
Host Cell	HCT116
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	FANCM Fanconi anemia, complementation group M [ Homo sapiens ]
Gene Symbol	FANCM
Synonyms	FAAP250; KIAA1596
Gene Description	Fanconi anemia, complementation group M
Gene ID	57697
Uni Prot ID	Q8IYD8
m RNA Refseq	NM_020937.2
Protein Refseq	NP_065988.1
Chromosome Location	14q21.2
Function	ATP binding; ATP-dependent helicase activity; DNA binding; chromatin binding; nuclease activity; protein binding;
Pathway	DNA Repair, organism-specific biosystem; FA core complex, organism-specific biosystem; Fanconi Anemia pathway, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem;
MIM	609644

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