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Human EFNB2 Stable Cell Line - HEK293T

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO01377

Host Cell :   HEK293T Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No. CSC-RO01377
Description This cell line is engineered to stably express Homo sapiens (human) ephrin B2 (EFNB2) in Human embryonic kidney immortal cell line transformed with SV40 large T antigen (HEK293T). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells.
Product Type Human gene overexpression stable cell line
Target Gene EFNB2
Gene Species Homo sapiens (human)
Host Cell HEK293T
Host Cell Species Homo sapiens (Human)
Reporter GFP
Applications 1) investigation of gene function
2) screening and validation of antibodies
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Quality Control 1) Real-time qPCR analysis of gene mRNA overexpression level
2) GFP fluorescent detection under fluorescent microscopy
3) mycoplasma detection
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Target Gene EFNB2
Background This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors. [provided by RefSeq, Jul 2008]
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We will definitely order from Creative Biogene again.

This EFNB2 stable HEK293T line has been a fantastic tool for studying Eph-Ephrin interactions. The surface presentation of the protein is high, and the cells are very easy to work with. It has provided us with consistent and high-quality data for our receptor binding studies.

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