Human DHX9 Stable Cell Line - HCT 116

The DHX9 gene encodes a member of the DExH-box family of helicases, also known as RNA helicase A (RHA) or nuclear DNA helicase II. This multifunctional, ATP-dependent helicase can untie DNA and RNA double strands and participates in almost all stages of nucleic acid metabolism, including transcriptional regulation, mRNA splicing, RNA transport, and translation. DHX9 also plays a crucial role in maintaining genome stability by resolving R loops (DNA-RNA hybrids) and promoting the repair of double-strand breaks. Clinically, DHX9 is frequently overexpressed in various human malignancies, such as breast cancer, hepatocellular carcinoma, and colorectal cancer, and its high expression is associated with advanced tumor stage and low patient survival. Conversely, heterozygous loss-of-function variants of DHX9 have recently been found to be associated with neurodevelopmental disorders characterized by intellectual disability and hypotonia. In cancer cells, DHX9 is essential for meeting the increasing demands of protein synthesis and preventing the accumulation of toxic nucleic acid structures, making it a highly attractive therapeutic target.

The stable human DHX9 cell line in the HCT 116 context is an ideal model for studying the roles of RNA and DNA helicases in cancer cell proliferation, genome maintenance, and therapeutic response. HCT 116 is a mature colorectal cancer cell line commonly used to study DNA repair mechanisms and the development of metabolic and molecular therapies. This cell line provides a stable platform for human DHX9 expression, enabling researchers to analyze how changes in helicase activity affect cells'' ability to cope with replication stress and their sensitivity to small molecule inhibitors. This product is widely used for high-throughput screening of DHX9-specific inhibitors and for validating the efficacy of combination therapies containing conventional chemotherapy drugs or DNA damage response (DDR) inhibitors. The stability of gene expression in this cell line ensures high reproducibility for long-term functional analyses and xenograft studies.