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Human DEGS1 KO Cell Line-HEK293T

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT2876

Target Gene :   DEGS1 Host Cell :   HEK293T

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No. CSC-RT2876
Description This cell is a stable cell line with a homozygous knockout of human DEGS1 using CRISPR/Cas9.
Target Gene DEGS1
Host Cell HEK293T
Host Cell Species Homo sapiens (Human)
Size 1 vial (>10^6 cell/vial)
Validation Sequencing
Storage Liquid Nitrogen
Shipping Dry ice package
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name DEGS1 delta-desaturase, sphingolipid 1 [ Homo sapiens ]
Gene Symbol DEGS1
Synonyms DEGS1; delta-desaturase, sphingolipid 1; degenerative spermatocyte homolog 1, lipid desaturase (Drosophila); sphingolipid delta-desaturase DES1; DEGS 1; Des 1; DES1; FADS7; MLD; sphingolipid delta desaturase 1; membrane lipid desaturase; dihydroceramide desaturase; sphingolipid delta 4 desaturase; migration-inducing gene 15 protein; sphingolipid delta-desaturase 1; membrane fatty acid (lipid) desaturase; cell migration-inducing gene 15 protein; degenerative spermatocyte homolog, lipid desaturase; degenerative spermatocyte homolog 1, lipid desaturase; DEGS; Des-1; MIG15; DEGS-1; MGC5079;
GeneID 8560
Uni ProtID O15121
mRNA Refseq BC000961
Chromosome Location 1q41-q42.12
Function electron carrier activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen;
Pathway Ceramide biosynthesis, organism-specific biosystem; Ceramide biosynthesis, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Sphingolipid de novo biosynthesis, organism-specific biosystem; Sphingolipid metabolism, organism-specific biosystem;
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