Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT2876
Target Gene : DEGS1 Host Cell : HEK293T
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT2876 |
| Description | This cell is a stable cell line with a homozygous knockout of human DEGS1 using CRISPR/Cas9. |
| Target Gene | DEGS1 |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Size | 1 vial (>10^6 cell/vial) |
| Validation | Sequencing |
| Storage | Liquid Nitrogen |
| Shipping | Dry ice package |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | DEGS1 delta-desaturase, sphingolipid 1 [ Homo sapiens ] |
| Gene Symbol | DEGS1 |
| Synonyms | DEGS1; delta-desaturase, sphingolipid 1; degenerative spermatocyte homolog 1, lipid desaturase (Drosophila); sphingolipid delta-desaturase DES1; DEGS 1; Des 1; DES1; FADS7; MLD; sphingolipid delta desaturase 1; membrane lipid desaturase; dihydroceramide desaturase; sphingolipid delta 4 desaturase; migration-inducing gene 15 protein; sphingolipid delta-desaturase 1; membrane fatty acid (lipid) desaturase; cell migration-inducing gene 15 protein; degenerative spermatocyte homolog, lipid desaturase; degenerative spermatocyte homolog 1, lipid desaturase; DEGS; Des-1; MIG15; DEGS-1; MGC5079; |
| GeneID | 8560 |
| Uni ProtID | O15121 |
| mRNA Refseq | BC000961 |
| Chromosome Location | 1q41-q42.12 |
| Function | electron carrier activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; |
| Pathway | Ceramide biosynthesis, organism-specific biosystem; Ceramide biosynthesis, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Sphingolipid de novo biosynthesis, organism-specific biosystem; Sphingolipid metabolism, organism-specific biosystem; |
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