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Human CSF2 Stable Cell Line - HuH-7

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO01328

Host Cell :   Huh7 Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RO01328
Description This cell line is engineered to stably express Homo sapiens (human) colony stimulating factor 2 (CSF2) in Human hepatocellular carcinoma / hepatoma / liver cancer cell line (HuH-7). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells.
Product Type Human gene overexpression stable cell line
Target Gene CSF2
Gene Species Homo sapiens (human)
Host Cell Huh7
Host Cell Species Homo sapiens (Human)
Reporter GFP
Applications 1) investigation of gene function
2) screening and validation of antibodies
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Quality Control 1) Real-time qPCR analysis of gene mRNA overexpression level
2) GFP fluorescent detection under fluorescent microscopy
3) mycoplasma detection
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Target Gene CSF2
Background The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. This gene plays a role in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been detected in SARS-CoV-2 infected patients that develop acute respiratory distress syndrome. Mice deficient in this gene or its receptor develop pulmonary alveolar proteinosis. [provided by RefSeq, Aug 2020]
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Work well

This CSF2 (GM-CSF) stable HuH-7 line shows excellent secretion levels of the target cytokine. We used it for co-culture experiments and found the cells to be extremely healthy and the results highly reproducible.

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