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Human CHST1 Stable Cell Line - TE-1

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO02559

Host Cell :   TE-1 Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RO02559
Description This cell line is engineered to stably overexpress Human CHST1 in TE-1 cell line. GFP reporter gene is also expressed in this cell line for cell tracking.
Product Type Stable cell line constitutively expressing target gene
Target Gene CHST1
Gene Species Human
Host Cell TE-1
Host Cell Species Homo sapiens (Human)
Applications Gene function studies and others
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Quality Control 1) Detection of gene overexpression by Real-time qPCR ,or western blot, or flow cytometry,
2) Mycoplasma detection
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Target Gene CHST1
Background This locus encodes a member of the keratin sulfotransferase family of proteins. The encoded enzyme catalyzes the sulfation of the proteoglycan keratin. [provided by RefSeq, Aug 2011]
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