Human CDC25A KO Cell Line-HeLa

Name: Human CDC25A KO Cell Line-HeLa
SKU: CSC-RT2995
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2995

Target Gene : CDC25A Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2995
Description	This cell is a stable cell line with a homozygous knockout of human CDC25A using CRISPR/Cas9
Target Gene	CDC25A
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CDC25A cell division cycle 25 homolog A (S. pombe) [ Homo sapiens ]
Gene Symbol	CDC25A
Synonyms	CDC25A2
Gene Description	cell division cycle 25 homolog A (S. pombe)
GeneID	993
Uni ProtID	P30304
mRNA Refseq	NM_001789.2
Protein Refseq	NP_001780.2
Chromosome Location	3p21
Function	protein binding; protein kinase binding; protein tyrosine phosphatase activity;
Pathway	Activation of ATR in response to replication stress, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem;
MIM	116947

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