Human ASS1 Knockout Cell Line-HEK293T

Name: Human ASS1 Knockout Cell Line-HEK293T
SKU: CSC-RT2801
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2801

Target Gene : ASS1 Host Cell : HEK293T

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2801
Description	This cell is a stable cell line with a homozygous knockout of human ASS1 using CRISPR/Cas9.
Target Gene	ASS1
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ASS1 argininosuccinate synthase 1 [ Homo sapiens ]
Gene Symbol	ASS1
Synonyms	ASS; CTLN1
Gene Description	argininosuccinate synthetase 1
GeneID	445
Uni ProtID	P00966
mRNA Refseq	NM_000050.4
Protein Refseq	NP_000041.2
Chromosome Location	9q34.1
Function	ATP binding; argininosuccinate synthase activity; protein binding; toxin binding;
Pathway	Alanine and aspartate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem;
MIM	603470

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