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Human APPswe Stable Cell Line - SH-SY5Y

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO01402

Host Cell :   SH-SY5Y Size :   >1x106 frozen cells/vial

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Cell Culture Information

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Cat. No. CSC-RO01402
Description This cell line is engineered to stably express Homo sapiens (human) amyloid beta precursor protein (APPswe) in Human neuroblastoma cell line (SH-SY5Y). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells.
Product Type Human gene overexpression stable cell line
Target Gene APPswe
Gene Species Homo sapiens (human)
Host Cell SH-SY5Y
Host Cell Species Homo sapiens (Human)
Reporter GFP
Applications 1) investigation of gene function
2) screening and validation of antibodies
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Quality Control 1) Real-time qPCR analysis of gene mRNA overexpression level
2) GFP fluorescent detection under fluorescent microscopy
3) mycoplasma detection
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
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Reliable and consistent data

We use this LN-229 cell line for our glioblastoma extracellular vesicle research. The CD9 overexpression is incredibly stable, which has vastly improved our exosome tracking and isolation workflows.

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