Human ACTA1 KO Cell Line-Hela

Name: Human ACTA1 KO Cell Line-Hela
SKU: CSC-RT2894
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2894

Target Gene : ACTA1 Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2894
Description	This cell is a stable cell line with a homozygous knockout of human ACTA1 using CRISPR/Cas9.
Target Gene	ACTA1
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ACTA1 actin, alpha 1, skeletal muscle [ Homo sapiens ]
Gene Symbol	ACTA1
Synonyms	ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
Gene Description	actin, alpha 1, skeletal muscle
GeneID	58
Uni ProtID	P68133
mRNA Refseq	NM_001100.3
Protein Refseq	NP_001091.1
Chromosome Location	1q42.13
Function	ADP binding; ATP binding; myosin binding; protein binding; structural constituent of cytoskeleton;
Pathway	Caspase cascade in apoptosis, organism-specific biosystem; Hypothetical Network for Drug Addiction, organism-specific biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem; RhoA signaling pathway, organism-specific biosystem; Signaling events mediated by focal adhesion kinase, organism-specific biosystem; Striated Muscle Contraction, organism-specific biosystem;
MIM	102610

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