GFP/OVAL Stable Cell Line - NCI-H520

Name: GFP/OVAL Stable Cell Line - NCI-H520
SKU: CSC-RO01881

Cat.No. : CSC-RO01881 Host Cell: NCI-H520

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Cell Line Information

Safety and Packaging

Cat. No.	CSC-RO01881
Description	This cell line is engineered to stably co-express GFP reporter gene and Chicken Ovalbumin (OVAL) in NCI-H520.
Target Gene	GFP/OVAL
Gene Species	Chicken
Host Cell	Human non-small cell lung cancer cell line
Species	Human cell line
Stability	This cell line is stable at least 10 passages.
Quality Control	1) Real-time qPCR analysis of OVAL mRNA overexpression level 2) Western blot analysis of OVAL protein overexpression level 3) Flow cytometry analysis of GFP expression 4) mycoplasma detection
Size Form	One vial of frozen cells, typically >1x10^6cells/vial
Storage	Liquid nitrogen
Shipping	Dry ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

Applications

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Q & A

Customer Reviews

Chicken ovalbumin (OVAL) is a major protein found in egg white, accounting for about 54% of the total protein content. It is a glycoprotein and plays several crucial roles, both in the biology of the egg and in various applications. 

Ovalbumin is a globular protein with a molecular weight of approximately 45 kDa. Its structure consists of a single polypeptide chain and is characterized by the presence of a folded domain with several alpha-helices and beta-sheets. 

Ovalbumin is also recognized as a significant allergen, particularly in children. It is commonly used in research to study allergic reactions and immune responses. Its role as a model protein in immunology makes it an important subject in allergy studies. Ovalbumin can also be used in vaccine development and as a model antigen in immunological studies. Understanding ovalbumin's characteristics and roles underscores its importance not only in biology but also in multiple industrial and research contexts.

Chicken ovalbumin (OVAL), a major protein found in egg whites, has been investigated for its potential applications in cancer research, particularly in cancer therapeutics and diagnostics. Its expression in cancer cell lines can provide insights into several key areas:

Target Antigen for Immunotherapy: OVAL can serve as a model antigen in cancer immunotherapy studies. By expressing OVAL in cancer cell lines, researchers can evaluate how the immune system recognizes and responds to tumor cells that express this protein. This approach can help in developing vaccines or immune checkpoint inhibitors.

Protein Isolation and Characterization: Expressing OVAL in cancer cell lines allows researchers to study its functional properties in a mammalian system. This helps in understanding how OVAL interacts with other cellular components and its potential role in cancer cell biology.

Cell Proliferation and Migration Studies: Investigating the effects of OVAL expression on cancer cell lines can reveal its role in cell proliferation, survival, and migration. These studies can provide insights into how this protein influences tumor progression and metastasis.

Drug Development: OVAL-expressing cancer cell lines can be utilized to screen for new chemotherapeutic agents or to study the efficacy of existing drugs. By observing how these drugs affect OVAL-expressing cells, researchers can determine whether OVAL impacts drug sensitivity or resistance.

Gene Therapy Approaches: If researchers can manipulate the expression of OVAL in cancer cells, they could potentially use it as a vehicle for targeted gene therapy, directing therapeutic agents specifically to tumor cells that express this protein.

Study of Post-Translational Modifications: The expression of OVAL in cancer cell lines allows for the investigation of post-translational modifications that may occur as a result of cancer cell metabolism or the tumor microenvironment.

The application of chicken ovalbumin expression in cancer cell lines is multifaceted, encompassing immunology, biomarker discovery, therapeutic development, and basic research into cancer biology. Further research in this area could lead to novel strategies for the diagnosis and treatment of cancer.

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