ETFDH Easy KO Kit (Human)

Name: ETFDH Easy KO Kit (Human)
SKU: CC-478
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-478

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Product Information

Gene Information

Cat. No.	CC-478
Description	A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Gene Abbr	Etfdh
Gene Name	ETFDH electron-transferring-flavoprotein dehydrogenase [ Homo sapiens ]
Species	Human
Ensembl ID	ENSMUSG00000027809
NCBIGene ID	66841
Uni Prot ID	Q921G7
Features	All-in-One workflow from gene editing to knockout validation for users with no prior experience. Pre-validated sgRNAs and primers for rapid setup. Streamlined experiment handling. CRISPR RNP method ensures precise and efficient gene knockout.
Applications	This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Reactions	5–10 reactions per target gene
Kit Components	2–3 chemically synthesized sgRNAs (200pmol each) 3 PCR/Sequencing primers (500pmol each) LM cell lysate (500µL) Cas9 protein (12µg) LM RNP transfection reagent (50µL)
Storage	Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.

Gene Name	ETFDH electron-transferring-flavoprotein dehydrogenase [ Homo sapiens ]
Gene Symbol	ETFDH
Synonyms	MADD; ETFQO
Gene Description	electron-transferring-flavoprotein dehydrogenase
Gene ID	2110
Uni Prot ID	Q16134
m RNA Refseq	NM_004453.2
Protein Refseq	NP_004444.2
Chromosome Location	4q32-q35
Function	4 iron, 4 sulfur cluster binding; electron carrier activity; electron-transferring-flavoprotein dehydrogenase activity; flavin adenine dinucleotide binding; metal ion binding; oxidoreductase activity; oxidoreductase activity, oxidizing metal ions with flavin as acceptor; quinone binding; ubiquinone binding;
Pathway	Metabolism, organism-specific biosystem; Respiratory electron transport, organism-specific biosystem; Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins., organism-specific biosystem; The citric acid (TCA) cycle and respiratory electron transport, organism-specific biosystem;
MIM	231675

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