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CC-1640

CRISPR Guide RNA in vitro Transcription Kit

For research use only. Not intended for any clinical use.

Cat. No. :   CC-1640

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Product Information

Cat. No. CC-1640
Description Contains T7 RNA Polymerase Mix for efficient transcription of CRISPR gRNA from minimal template. Suitable for Cas12a, Cas13a, Cas12b, Cas9 gRNA synthesis. 0.5 μg template yields up to ~100 μg gRNA per reaction.
Gene Abbr Multiple
Features High transcription efficiency, high yield
Applications gRNA preparation
Manipulation In vitro transcription, incubate at 37°C for 1–2 h, purify RNA
Reactions 96T
Kit Components T7 Reaction Buffer (10X), T7 RNA Polymerase Mix, NTP Mix (25 mM each), Positive Control, DNase I Buffer (10X), DNase I, RNase Inhibitor, Purification Buffer, RNase-free ddH2O
Storage Store at -20°C, avoid repeated freeze-thaw
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