Chicken OVAL/Luc Stable Cell Line - B16F10

Name: Chicken OVAL/Luc Stable Cell Line - B16F10
SKU: CSC-RO01303
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO01303

Host Cell : B16F10 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO01303
Description	This cell line is engineered to stably express chicken ovalbumin (OVA, OVAL, SERPINB14) and luciferase reporter gene in B16F10 cells.
Product Type	Constitutive gene expression stable cell line
Target Gene	OVAL/Luc
Gene Species	Gallus gallus domesticus (Chicken)
Host Cell	B16F10
Host Cell Species	Mus musculus (Mouse)
Reporter	Luciferase reporter gene
Applications	1) in vitro cell-based assays 2) in vivo tumor growth studies
Size	One vial of frozen cells, typically >1x10^6cells/vial
Stability	This cell line is stable at least 10 passages.
Quality Control	1) Gene expression assay 2) Mycoplasma detection
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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