Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RO01316
Host Cell : Hepa1-6 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RO01316 |
| Description | This cell line is engineered to stably express Gallus gallus (chicken) ovalbumin (SERPINB14) (OVAL) and Cas9 in Mouse liver cancer / hepatocellular carcinoma cell line (Hepa 1-6). |
| Product Type | Chicken gene overexpression stable cell line |
| Target Gene | OVAL and Cas9 |
| Gene Species | Gallus gallus (chicken) |
| Host Cell | Hepa1-6 |
| Host Cell Species | Mus musculus (Mouse) |
| Applications |
1) CRISPR genome editing, such as gene knockout (KO), gene knockin (KI), gene mutagenesis, gene tagging etc. 2) High-throughput sgRNA screening and validation 3) Anti-cancer drug development |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Quality Control |
1) Real-time qPCR analysis of gene mRNA overexpression level 2) mycoplasma detection |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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The dual-expression of OVAL and Cas9 in the Hepa 1-6 line is a fantastic resource. It allowed us to perform CRISPR modifications and track immune responses simultaneously. The stability of both inserts is impressive, making it perfect for syngeneic mouse models.
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