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Chicken OVAL and Cas9 Stable Cell Line - Hepa 1-6

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO01316

Host Cell :   Hepa1-6 Size :   >1x106 frozen cells/vial

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Cat. No. CSC-RO01316
Description This cell line is engineered to stably express Gallus gallus (chicken) ovalbumin (SERPINB14) (OVAL) and Cas9 in Mouse liver cancer / hepatocellular carcinoma cell line (Hepa 1-6).
Product Type Chicken gene overexpression stable cell line
Target Gene OVAL and Cas9
Gene Species Gallus gallus (chicken)
Host Cell Hepa1-6
Host Cell Species Mus musculus (Mouse)
Applications 1) CRISPR genome editing, such as gene knockout (KO), gene knockin (KI), gene mutagenesis, gene tagging etc.
2) High-throughput sgRNA screening and validation
3) Anti-cancer drug development
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Quality Control 1) Real-time qPCR analysis of gene mRNA overexpression level
2) mycoplasma detection
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
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Accelerated our research progress

The dual-expression of OVAL and Cas9 in the Hepa 1-6 line is a fantastic resource. It allowed us to perform CRISPR modifications and track immune responses simultaneously. The stability of both inserts is impressive, making it perfect for syngeneic mouse models.

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