Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CC-1624
| Cat. No. | CC-1624 |
| Description | DNA template with T7 promoter is transcribed into RNA by T7 RNA polymerase. Cas13a forms a complex with crRNA, pairs with target RNA, and is specifically activated to trans-cleave fluorescent reporters. This kit uses upgraded LwaCas13a protein with 50% higher activity than wild type, enhancing detection performance. |
| Gene Abbr | Cas13a |
| Features | 50% increased activity |
| Applications | Molecular diagnostics, pathogen detection |
| Manipulation | Thaw on ice, mix reagents. Prepare 20 μL reaction, incubate at 37°C for 30-60 min, dilute and apply to lateral flow strip |
| Reactions | 96T |
| Kit Components | Cleavage Buffer (10X), Trans Mix (5X), T7 RNA Polymerase (40X), Cas13a Protein (10 μM), Cas13a Diluent Buffer, Positive Control (10X), Reporter (4 μM), crRNA for Positive Control (20X), Diluent Buffer |
| Storage | Store at -20°C |
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