pMito-iRFP713

The pMito-iRFP713 vector is widely used in various applications of scientific research, particularly in the field of cellular and molecular biology. Here are some of the main areas of its application:

Mitochondrial Imaging: The pMito-iRFP713 vector is designed to express iRFP713 that is localized to the mitochondria. This means that it helps in visualizing mitochondria in live cells through fluorescence microscopy, or any other imaging techniques requiring a far-red to near-infrared fluorescent protein.

Cell Biology: It is commonly used in a variety of cellular studies, such as tracking cell division, differentiation, and function, analyzing mitochondrial dynamics and function, studying cell proliferation, apoptosis, migration, invasion, etc.

Gene Expression Studies: The vector can be used to study mitochondrial genes' expression and regulation. By tagging a gene of interest with iRFP713, it's possible to study the gene's activity within the context of the entire cell or organism.

Protein Localization Studies: It can be used for protein localization studies. It enables the exploitation of the near-infrared (NIR) spectral window optimal for in vivo imaging.

Genetic Engineering: The vector plays a crucial role in the creation of genetically modified organisms (GMOs). Scientists can use this vector to import desired genes into target cells.