Pages
Products

pMito-iRFP713

For research use only. Not intended for any clinical use.
Cat.No.
OVT2737
Host Cell
Mammalian cells, Escherichia coli
Product Type
Vector
Promoter
CMV
Resistance
Kanamycin
Selection
Neomycin
Vector Size
4989 bp

Background

Applications

Publications

Q & A

Customer Reviews

The pMito-iRFP713 vector is a subcellular localization vector with a size of 4989 base pairs (bp). This particular vector is designed to provide an advanced tool for studying the dynamics of mitochondrial processes in various cellular contexts. It employs the use of the iRFP713 gene as a reporter. iRFP713 is a near-infrared fluorescent protein that emits fluorescence in the near-infrared region of the spectrum, which permits deep imaging in tissue. This feature makes the pMito-iRFP713 vector an invaluable tool for in-vivo imaging studies of mitochondrial behaviors. The pMito-iRFP713 vector contains the Cytomegalovirus (CMV) promoter, a strong, constitutive promoter commonly used in mammalian expression vectors due to its ability to initiate high-level expression of downstream genes. The CMV promoter ensures a robust expression of the iRFP713 gene in virtually all cell types. Hence, it permits a better visualization of mitochondrial processes.
The pMito-iRFP713 vector is widely used in various applications of scientific research, particularly in the field of cellular and molecular biology. Here are some of the main areas of its application: Mitochondrial Imaging: The pMito-iRFP713 vector is designed to express iRFP713 that is localized to the mitochondria. This means that it helps in visualizing mitochondria in live cells through fluorescence microscopy, or any other imaging techniques requiring a far-red to near-infrared fluorescent protein. Cell Biology: It is commonly used in a variety of cellular studies, such as tracking cell division, differentiation, and function, analyzing mitochondrial dynamics and function, studying cell proliferation, apoptosis, migration, invasion, etc. Gene Expression Studies: The vector can be used to study mitochondrial genes' expression and regulation. By tagging a gene of interest with iRFP713, it's possible to study the gene's activity within the context of the entire cell or organism. Protein Localization Studies: It can be used for protein localization studies. It enables the exploitation of the near-infrared (NIR) spectral window optimal for in vivo imaging. Genetic Engineering: The vector plays a crucial role in the creation of genetically modified organisms (GMOs). Scientists can use this vector to import desired genes into target cells.
Customer Q&As
What is the species source of the iRFP713?

A: The iRFP713 is sourced from the Rhodopseudomonas palustris.

How does iRFP713 photostability compare to EYFP?

A: iRFP713 shows superior photostability compared to EYFP.

How do electrophysiological recordings from iRFP713-labeled cells compare to cells labeled with mCherry?

A: Electrophysiological recordings from iRFP713-labeled cells suggest that they are healthier and therefore more stable and reliable in an ex vivo preparation compared to cells labeled with mCherry.

Does iRFP713 require exogenously supplied chromophore biliverdin for detection?

A: No, iRFP713 is detectable without exogenously supplied chromophore biliverdin.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Customer Reviews
Highly effective tool

The pMito-iRFP713 vector is a highly effective tool in cellular and mitochondrial research. The infrared fluorescent protein, iRFP713, allows for non-invasive visualization of mitochondrial dynamics in live cells.

Canada

05/23/2022

An excellent choice

The pMito-iRFP713 is in essence designed for optimal expression and localization to the mitochondria, making it an excellent choice for researchers interested in studying intracellular processes such as mitochondrial biogenesis and autophagy.

United States

03/28/2021

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

CBpromise

Our promise to you:
Guaranteed product quality, expert customer support.

24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Quick Inquiry

Inquiry