pCDH-CMV-MCS-EF1-GFP-Puro

The pCDH-CMV-MCS-EF1-GFP-Puro vector has valuable applications in gene cloning, gene expression and stable gene transfer. This lentiviral expression system combines the benefits of multi-cloning sites, strong promoter for heterogeneous expression, green fluorescent protein (GFP) and puromycin resistance gene for selection purpose.

Gene Cloning: The multi-cloning sites available in the vector allow scientists to insert and clone any gene of interest quickly and precisely.

Gene Expression: The strong cytomegalovirus (CMV) promoter facilitates high-level transient expression of cloned genes in a wide range of mammalian cells.

Tracing Gene Expression: This vector comes with GFP reporter, allowing researchers to conveniently observe and trace the expression and location of inserted genes under a fluorescence microscope.

Stable Gene Transfer: This lentiviral system operates in both dividing and non-dividing cells, which permits stable gene transfer and consistent expression.

Selection Marker: The puromycin resistance gene helps in selection and maintenance of cells that have incorporated the vectors. This can be done by applying puromycin to the culture medium. Only cells that contain the vector will survive, simplifying the selection process.

Thus, the pCDH-CMV-MCS-EF1-GFP-Puro vector serves as a versatile tool in various molecular biology applications, helping scientists to better understand gene functions, promote drug discovery and development, and explore the potential of gene therapy.