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pSilencer 4.1-CMV neo

For research use only. Not intended for any clinical use.
Cat.No.
OVT2562
Promoter
CMV
Selection
Ampicillin, Neomycin
Source
Mammalian, Escherichia coli
Species
Bacterial

Background

Applications

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Q & A

Customer Reviews

The pSilencer 4.1-CMV siRNA Expression Vectors are advanced tools used in molecular biology studies, known for their competence in facilitating directional cloning. The process involves the vectors being linearized with both the BamH 1 and Hind III enzymes, a feature that enables them to provide greater cloning efficiency and simplicity. An additional feature of the pSilencer 4.1-CMV vectors is their purification process. This process eradicates the digested insert, thereby preventing it from re-ligating with the vector. In eliminating this possibility, the percentage of clones bearing the hairpin siRNA template insert significantly increases after the ligation process. This advancement not only simplifies the procedure but also cuts down both the time and effort essential for screening clones. Furthermore, this vector has a high copy number, making it particularly convenient for producing abundant plasmid DNA. The promoter or the genetic sequence of the vector that initiates transcription is the Cytomegalovirus (CMV), which is renowned for its strong, constitutive expression in an array of mammalian cells.
pSilencer 4.1-CMV neo vector is a commonly used tool in molecular biology research, particularly in gene expression studies and gene therapy. Gene Silencing: The pSilencer 4.1-CMV neo vector is utilized to introduce specific short interfering RNA (siRNA) sequences into cells to knockdown the expression of a particular gene. These siRNA molecules work by destroying specific mRNA molecules, reducing the protein’s production. This aids in studying the function of specific genes and the proteins they produce. Gene Expression: The vector can be used to carry a target gene into the cells. By incorporating the required genes into the vector and introducing it to the host cells, researchers can examine how the gene behaves under certain conditions or how it interacts with other genes. Production of Recombinant Proteins: The vector is also used to produce certain proteins in the laboratory. By inserting the gene for a specific protein into the vector, one can create proteins that don't naturally occur in the cells, which can be useful for treatments, research, and development of new drugs. Gene Therapy: Increasingly, vectors like pSilencer 4.1-CMV neo are being used in gene therapy for treating genetic diseases.
Customer Q&As
How are the pSilencer 4.1-CMV neo vectors linearized and purified?

A: The pSilencer 4.1-CMV neo Expression vectors are linearized with BamH 1 and Hind III to facilitate directional cloning. They are also purified to remove the digested insert.

Why is the linearization and purification of the vectors important?

A: This process prevents the digested insert from re-ligating with the vector, increasing the percentage of clones bearing the hairpin siRNA template insert after ligation. This reduces the time and effort required to screen clones.

How does the neomycin resistance gene facilitate long-term gene knockdown studies?

A: The neomycin resistance gene allows for the selection of cell populations or clonal cell lines that stably express the hairpin siRNA.

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Customer Reviews
Highly efficient

The pSilencer 4.1-CMV neo vector is highly efficient for directional cloning due to its linearization with BamH 1 and Hind III. This feature significantly reduces the time and effort required to screen clones bearing the hairpin siRNA template insert after ligation.

United States

01/27/2022

Worked very well

The neomycin resistance gene in the pSilencer 4.1-CMV neo vector also enables long-term gene knockdown studies. This feature provides us with a powerful tool to study and manipulate target gene expression over an extended period.

United States

07/19/2022

Excellent tool

The pSilencer 4.1-CMV neo vector is an excellent tool for gene silencing research. It offers high-efficiency siRNA expression, resulting in robust knockdown of target gene expression.

United Kingdom

02/11/2021

Simplified process

The pSilencer 4.1-CMV neo vector significantly simplifies the process of identifying and isolating successfully transfected cells, thereby saving both the effort and time.

French

04/07/2020

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