Mouse Slc7a9 Stable Cell Line - HEK293

Name: Mouse Slc7a9 Stable Cell Line - HEK293
SKU: CSC-SC014652-MH
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC014652-MH

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Cat. No.	CSC-SC014652-MH
Description	This cell line is engineered to stably overexpress mouse Slc7a9 with C-teminal FLAG tag.
Target Gene	Slc7a9
Gene Species	Mus musculus (Mouse)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

Applications

Q & A

Customer Reviews

The Mouse Slc7a9 gene, also known as xCT, encodes a subunit of the cystine-glutamate antiporter system xc(-). This transporter plays a critical role in the regulation of intracellular glutathione levels and the maintenance of redox balance. It is involved in the uptake of cystine, which is then used to synthesize glutathione, an important antioxidant. The Slc7a9 gene is highly expressed in various tissues, including the liver, and its dysregulation has been associated with diseases such as cancer and neurological disorders.

The HEK293 cell line, derived from human embryonic kidney cells, has been engineered to stably express the Mouse Slc7a9 gene. This stable cell line serves as an excellent model for studying the function of xCT in cellular redox balance and its role in disease pathogenesis. Researchers can use this cell line to investigate the effects of Slc7a9 expression on cellular stress responses, its interaction with other antioxidants, and its potential as a therapeutic target for diseases where redox balance is disrupted.

The Mouse Slc7a9 Stable Cell Line - HEK293 is a cell line engineered to express the Slc7a9 gene from mice, which encodes for the large neutral amino acid transporter (LAT1). This transporter is involved in the uptake of amino acids, which are essential for protein synthesis and cell growth. The applications of this cell line include:

(1)Cancer Research: LAT1 is often overexpressed in various types of cancer cells, making it a potential target for cancer therapy. This cell line can be used to study the role of LAT1 in tumor growth and to test the efficacy of drugs that target this transporter.

(2)Drug Development: Researchers can use the Mouse Slc7a9 Stable Cell Line to screen for compounds that inhibit LAT1 activity, which could lead to the development of new cancer treatments. This cell line provides a platform for understanding the transport mechanisms of amino acids and their role in cellular metabolism.

(3)Metabolism Studies: The expression of LAT1 allows for the investigation of amino acid metabolism and the role of this transporter in the regulation of cellular nutrient uptake. This can contribute to the understanding of metabolic disorders and the development of therapies for conditions such as obesity and diabetes.

Customer Q&As

What is the origin of the Mouse Slc7a9 Stable Cell Line - HEK293, and how was it established?

A: The Mouse Slc7a9 Stable Cell Line - HEK293 was derived from mouse tissues and established by transfecting the Slc7a9 gene into HEK293 (Human Embryonic Kidney 293) cells. HEK293 cells are a widely used human embryonic kidney cell line in biomedical research.

What is the expression level of the Slc7a9 gene in the Mouse Slc7a9 Stable Cell Line - HEK293?

A: The expression level of the Slc7a9 gene in the Mouse Slc7a9 Stable Cell Line - HEK293 is high. Transfection of the Slc7a9 gene results in the cells acquiring additional copies of the gene, thereby increasing its expression levels within the cells.

In what aspect does the Mouse Slc7a9 Stable Cell Line - HEK293 differ from other cell lines?

A: The main difference of the Mouse Slc7a9 Stable Cell Line - HEK293 from other cell lines lies in its stable expression of the Slc7a9 gene, making it an ideal model for studying Slc7a9 function and its regulation.

What are the structural characteristics of the Slc7a9 protein in the Mouse Slc7a9 Stable Cell Line - HEK293?

A: The Slc7a9 protein in the Mouse Slc7a9 Stable Cell Line - HEK293 is a transmembrane transporter protein with multiple transmembrane domains and intracellular and extracellular domains responsible for substance transport.

How is the Mouse Slc7a9 Stable Cell Line - HEK293 utilized in studying the function of the Slc7a9 gene?

A: The Mouse Slc7a9 Stable Cell Line - HEK293 can be used to study the function of the Slc7a9 gene, including its intracellular roles, interactions with other proteins, and regulation of physiological processes.

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Customer Reviews

Stable expression

The Mouse Slc7a9 Stable Cell Line-HEK293 provides sustained stable gene expression in HEK293 cells, suitable for long-term experiments and research.

Canada

2023-08-12

Efficient transfection

The cell line is easily transfected, ensuring high expression of the Slc7a9 gene, facilitating rapid experiment initiation.

French

2023-04-06

Reliable data

This product offers reliable experimental data, ensuring accuracy and reproducibility of experimental results.

Germany

2020-04-10

Excellent stability

Rigorously selected, the cell line demonstrates excellent stability, avoiding fluctuations in gene expression.

Canada

2020-04-16

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Mouse Slc7a9 Stable Cell Line - HEK293

Cell Line Information

Cell Culture Information

Safety and Packaging

Background

Applications

Q & A

Customer Reviews

What is the origin of the Mouse Slc7a9 Stable Cell Line - HEK293, and how was it established?

What is the expression level of the Slc7a9 gene in the Mouse Slc7a9 Stable Cell Line - HEK293?

In what aspect does the Mouse Slc7a9 Stable Cell Line - HEK293 differ from other cell lines?

What are the structural characteristics of the Slc7a9 protein in the Mouse Slc7a9 Stable Cell Line - HEK293?

How is the Mouse Slc7a9 Stable Cell Line - HEK293 utilized in studying the function of the Slc7a9 gene?

Stable expression

Efficient transfection

Reliable data

Excellent stability

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