Cat.No. : CSC-SC014652-MH Host Cell: HEK293
| Cat. No. | CSC-SC014652-MH |
| Description | This cell line is engineered to stably overexpress mouse Slc7a9 with C-teminal FLAG tag. |
| Gene | Slc7a9 |
| Gene Species | Mus musculus (Mouse) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Mouse Slc7a9 Stable Cell Line - HEK293 was derived from mouse tissues and established by transfecting the Slc7a9 gene into HEK293 (Human Embryonic Kidney 293) cells. HEK293 cells are a widely used human embryonic kidney cell line in biomedical research.
A: The expression level of the Slc7a9 gene in the Mouse Slc7a9 Stable Cell Line - HEK293 is high. Transfection of the Slc7a9 gene results in the cells acquiring additional copies of the gene, thereby increasing its expression levels within the cells.
A: The main difference of the Mouse Slc7a9 Stable Cell Line - HEK293 from other cell lines lies in its stable expression of the Slc7a9 gene, making it an ideal model for studying Slc7a9 function and its regulation.
A: The Slc7a9 protein in the Mouse Slc7a9 Stable Cell Line - HEK293 is a transmembrane transporter protein with multiple transmembrane domains and intracellular and extracellular domains responsible for substance transport.
A: The Mouse Slc7a9 Stable Cell Line - HEK293 can be used to study the function of the Slc7a9 gene, including its intracellular roles, interactions with other proteins, and regulation of physiological processes.
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The Mouse Slc7a9 Stable Cell Line-HEK293 provides sustained stable gene expression in HEK293 cells, suitable for long-term experiments and research.
The cell line is easily transfected, ensuring high expression of the Slc7a9 gene, facilitating rapid experiment initiation.
This product offers reliable experimental data, ensuring accuracy and reproducibility of experimental results.
Rigorously selected, the cell line demonstrates excellent stability, avoiding fluctuations in gene expression.
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