Cat.No. : CSC-RO0248 Host Cell: CHO-K1
| Cat. No. | CSC-RO0248 |
| Description | This cell line is egineered to stably overexpress mouse Clec9a with no tag. Overexpression of mouse Clec9a has been validated through real-time QPCR and FACS. |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Clec9a gene encodes for the CLEC9A protein, also known as C-type lectin domain family 9A. This protein is a member of the C-type lectin family, a group of carbohydrate-binding proteins involved in various biological processes. CLEC9A is expressed on the surface of dendritic cells and plays a role in the activation of innate immunity and the regulation of adaptive immune responses.
A: The Clec9a gene contributes to immune response by enabling dendritic cells to detect and respond to pathogens and tumor cells. CLEC9A binds to specific carbohydrate structures on the surface of these cells, triggering their activation and the subsequent production of pro-inflammatory cytokines. This process is crucial for the initiation and regulation of immune responses.
A: Mutations in the Clec9a gene are not commonly associated with specific diseases. However, dysfunctional CLEC9A expression or function may lead to alterations in immune responses, potentially affecting the body's ability to mount effective defenses against pathogens or cancer cells.
A: The expression of the Clec9a gene is regulated by various factors, including cytokines and pathogen-associated molecular patterns. The activation of dendritic cells by pathogens or tumor cells can lead to the upregulation of CLEC9A expression. Additionally, regulatory mechanisms, such as epigenetic modifications and post-translational modifications, can influence the expression of CLEC9A, ensuring proper immune responses.
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The Mouse Clec9a Stable Cell Line - CHO undergoes strict quality control measures, including mycoplasma testing and viability checks, ensuring that researchers receive a high-quality and reliable cell line for their experiments.
The cell line is provided with a well-established and efficient delivery system, enabling researchers to receive the cells in a timely manner while maintaining their viability and functionality during transit.
The Mouse Clec9a Stable Cell Line - CHO is shipped under optimal conditions, including temperature-controlled packaging and overnight delivery, to ensure the preservation of cell viability. Additionally, clear storage guidelines are provided to maintain cell line stability over time.
This cell line finds applications in various research areas, such as immunology, cancer biology, and drug discovery. It can be used for studying the function of Clec9a, developing antibody-based therapeutics, and assessing the immune response to potential therapeutic candidates.
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