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Human WNT5B Knockout Cell Line-HEK293T

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT3214

Target Gene :   WNT5B Host Cell :   HEK293T

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT3214
Introduction This cell is a stable cell line with a homozygous knockout of human WNT5B using CRISPR/Cas9.
Target Gene WNT5B
Host Cell HEK293T
Host Cell Species Homo sapiens (Human)
Size 1 vial (>10^6 cell/vial)
Validation Sequencing
Storage Liquid Nitrogen
Shipping Dry ice package
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Target Gene Wnt5b
Background Enables signaling receptor binding activity. Acts upstream of or within negative regulation of canonical Wnt signaling pathway and positive regulation of fat cell differentiation. Located in cell surface and extracellular matrix. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; limb; and sensory organ. Orthologous to human WNT5B (Wnt family member 5B). [provided by Alliance of Genome Resources, Feb 2025]
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