Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT3214
Target Gene : WNT5B Host Cell : HEK293T
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT3214 |
| Introduction | This cell is a stable cell line with a homozygous knockout of human WNT5B using CRISPR/Cas9. |
| Target Gene | WNT5B |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Size | 1 vial (>10^6 cell/vial) |
| Validation | Sequencing |
| Storage | Liquid Nitrogen |
| Shipping | Dry ice package |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Target Gene | Wnt5b |
| Background | Enables signaling receptor binding activity. Acts upstream of or within negative regulation of canonical Wnt signaling pathway and positive regulation of fat cell differentiation. Located in cell surface and extracellular matrix. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; limb; and sensory organ. Orthologous to human WNT5B (Wnt family member 5B). [provided by Alliance of Genome Resources, Feb 2025] |
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