Human SLC2A5 Stable Cell Line - CHO-K1

SLC2A5, also known as GLUT5 or GLUT-5, is a member of the solute carrier family 2 and encodes a facilitative fructose transporter. The gene is located on human chromosome 1p36.23 and is primarily expressed in the small intestine, duodenum, and to a lesser extent in the kidney, adipose tissue, and skeletal muscle. The GLUT5 protein mediates the uptake of fructose across the apical membrane of enterocytes and plays a key role in dietary fructose absorption. Dysregulation of SLC2A5 expression has been linked to metabolic disorders, hypertension, and certain cancers, making it an important target for research. The Human SLC2A5 Stable Cell Line - CHO-K1 is generated by stably transfecting the human SLC2A5 gene into the CHO-K1 host cell line. The stable integration ensures consistent and reproducible expression over multiple passages, which is critical for long-term experimental workflows.

This cell line is widely used in functional assays to characterize fructose transport activity and to evaluate potential inhibitors or modulators of GLUT5. Researchers can perform uptake assays using radiolabeled or fluorescent fructose analogs to measure transport kinetics, determine Michaelis-Menten parameters, and screen compound libraries for selective inhibitors. Given the growing interest in targeting GLUT5 for therapeutic intervention in conditions such as fructose malabsorption, metabolic syndrome, and GLUT5-overexpressing tumors, this stable cell line serves as a reliable tool for drug discovery and lead optimization. It can also be employed in mechanistic studies to investigate how mutations or post-translational modifications affect transporter trafficking, membrane localization, and activity. Additionally, the CHO-K1 background is well-suited for electrophysiological recordings and surface expression analysis, enabling a comprehensive understanding of GLUT5 biology. The combination of a defined genetic background and stable, high-level expression makes this cell line a practical and reproducible platform for both basic research and preclinical screening applications.