Human PIEZO1 Knockout Cell Line-A549

Name: Human PIEZO1 Knockout Cell Line-A549
SKU: CSC-RT2796
Rating: 4.0 (2 reviews)

Cat.No. : CSC-RT2796

Host Cell: A549 Target Gene: PIEZO1

Size: 1x10^6 cells/vial, 1mL Validation: Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RT2796
Description	This cell is a stable cell line with a homozygous knockout of human PIEZO1 using CRISPR/Cas9.
Target Gene	PIEZO1
Host Cell	A549
Host Cell Species	Homo sapiens (Human)
Size Form	1 vial (>10^6 cell/vial)
Shipping	Dry ice package
Storage	Liquid Nitrogen

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

Applications

Publications

Q & A

Customer Reviews

PIEZO1 is a gene that encodes a protein called piezoelectric mechanosensitive ion channel component 1. PIEZO1 protein is found in many tissues throughout the body, such as blood vessels, kidneys, lungs, and bladder. Structurally, PIEZO1 forms a three-bladed propeller-like structure, as revealed by crystallographic studies. This unique structure is essential for its function, enabling the channel to respond to mechanical stress by changing its conformation. Functionally, PIEZO1 acts as a pore-forming subunit of a mechanosensitive ion channel, allowing the influx of cations, such as calcium ions, upon mechanical stimulation. This gating mechanism has profound consequences for cell physiology, affecting processes as diverse as ion transport across membranes, regulation of cell adhesion, integrin activation, and cellular responses to mechanical stimulation.

PIEZO1 mutations are associated with various genetic diseases. For example, hereditary polycythemia (HX), a rare genetic disease characterized by dehydration of red blood cells. In patients with this disease, certain mutations in PIEZO1 cause overactive ion channels, which results in an imbalance in ion transport, leading to reduced cell size and increased red blood cell fragility. Clinical manifestations of HX include anemia, jaundice, and an increased risk of gallstones. In addition to inherited red blood cell disorders, studies have implicated PIEZO1 in other disorders, such as lymphatic dysplasia. Mutations in PIEZO1 can lead to dysfunction of lymphatic valves, which can result in improper lymphatic flow regulation and lymphedema. Additionally, there is emerging evidence that PIEZO1 may play a role in cancer metastasis, as its mechanosensitive properties affect how cancer cells behave as they migrate and invade new tissues.

Applications of Human PIEZO1 Knockout Cell Line-A549

Mechanotransduction Research: The PIEZO1 gene is known to encode a mechanosensitive ion channel involved in converting mechanical stimuli into electrochemical signals. By utilizing the Human PIEZO1 Knockout Cell Line-A549, researchers can study the specific role of PIEZO1 in mechanotransduction pathways, helping to elucidate its function and regulatory mechanisms in human cells.

Disease Modeling: PIEZO1 has been implicated in various diseases, including polycythemia vera and dehydrating hereditary stomatitis (DHS). The PIEZO1 knockout model provides an important tool to understand how loss of PIEZO1 function causes these diseases at the cellular level, which may lead to the development of new therapeutic strategies.

Cancer Research: PIEZO1 is often involved in tumor progression and metastasis. The Human PIEZO1 Knockout Cell Line-A549 is an important model for studying how PIEZO1 affects cancer cell migration, invasion, and response to mechanical changes in the tumor microenvironment.

Drug Screening: This knockout cell line can be used for high-throughput screening of drugs targeting mechanosensitive pathways. It enables researchers to identify and characterize new pharmacological agents that modulate PIEZO1 activity or compensate for its loss, potentially leading to new treatments for diseases associated with mechanotransduction dysfunction.

Cell Physiology Studies: PIEZO1 channels are integral to a variety of cellular physiological processes, including cell volume regulation, calcium signaling, and stress responses. By comparing cellular processes in PIEZO1 knockout cells and wild-type A549 cells, researchers can gain a deeper understanding of the physiological role of PIEZO1 and its contribution to overall cellular function.

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Reliable cell model

As a researcher focusing on mechanotransduction, the Human PIEZO1 Knockout Cell Line-A549 has been an invaluable asset to our lab. It has provided us with a reliable model to study the absence of PIEZO1 and its physiological roles.

United States

07/30/2024

Delivered on time and in excellent condition

The knockout cells were delivered on time and in excellent condition, making it easy to integrate them into our ongoing experiments.

Canada

01/23/2023

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Human PIEZO1 Knockout Cell Line-A549

Cell Line Information

Cell Culture Information

Safety and Packaging

Background

Applications

Publications

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Reliable cell model

Delivered on time and in excellent condition

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