Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RO1083
Host Cell : HEK293 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RO1083 |
| Description | HEK293-NRP2 cell line is engineered to stably overexpress human NRP2. |
| Target Gene | NRP2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications |
1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | NRP2 neuropilin 2 [ Homo sapiens ] |
| Gene Symbol | NRP2 |
| Synonyms | NP2; NPN2; PRO2714; VEGF165R2 |
| Gene Description | neuropilin 2 |
| GeneID | 8828 |
| Uni ProtID | O60462 |
| mRNA Refseq | NM_201279.1 |
| Protein Refseq | NP_958436.1 |
| Chromosome Location | 2q33.3 |
| Function | cytokine binding; growth factor binding; heparin binding; metal ion binding; receptor activity; semaphorin receptor activity; vascular endothelial growth factor-activated receptor activity; |
| Pathway | Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; L1CAM interactions, organism-specific biosystem; Neurophilin interactions with VEGF and VEGFR, organism-specific biosystem; NrCAM interactions, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by VEGF, organism-specific biosystem; |
| MIM | 602070 |
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