Human NPR2 Stable Cell Line - CHO

Name: Human NPR2 Stable Cell Line - CHO
SKU: CSC-SC010566-1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC010566-1

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Documents

Cat. No.	CSC-SC010566-1
Description	This cell line is engineered to stably express human natriuretic peptide receptor 2 (NPR2) in CHO-K1 cells.
Target Gene	NPR2
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	In vitro cell-based C-type natriuretic peptide (CNP)-targeted screening
Size	One vial of frozen cells, typically >1x10^6cells/vial
Stability	This cell line is stable at least 10 passages.
Quality Control	1) Real-Time qPCR, 2) Flow cytometry analysis, 3) Mycoplasma detection
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent cell line

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Target Gene

NPR2

Background

This gene encodes natriuretic peptide receptor B, one of two integral membrane receptors for natriuretic peptides. Both NPR1 and NPR2 contain five functional domains: an extracellular ligand-binding domain, a single membrane-spanning region, and intracellularly a protein kinase homology domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. The protein is the primary receptor for C-type natriuretic peptide (CNP), which upon ligand binding exhibits greatly increased guanylyl cyclase activity. Mutations in this gene are the cause of acromesomelic dysplasia Maroteaux type. [provided by RefSeq, Jul 2008]

CSC-SC010566-1-Human NPR2 Stable Cell Line - CHO-Datasheet

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