Human MTHFD2 Stable Cell Line - HEK293

Name: Human MTHFD2 Stable Cell Line - HEK293
SKU: CSC-SC009879-1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC009879-1

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Documents

Cat. No.	CSC-SC009879-1
Description	This cell line is engineered to stably overexpress human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2).
Target Gene	MTHFD2
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Investigation of human MTHFD2 function
Size	One vial of frozen cells, typically >1x10^6cells/vial
Stability	This cell line is stable at least 10 passages.
Quality Control	Analysis of human MTHFD2 overexpression level by QPCR Mycoplasma detection
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Target Gene

MTHFD2

Background

This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]

CSC-SC009879-1-Human MTHFD2 Stable Cell Line - HEK293-Datasheet

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