Human IAPP Stable Cell Line - HeLa

Name: Human IAPP Stable Cell Line - HeLa
SKU: CSC-SC007374-1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC007374-1

Host Cell : HeLa Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-SC007374-1
Description	This cell line is engineered to stably express human islet amyloid polypeptide (IAPP) in HeLa cells.
Target Gene	IAPP
Gene Species	Homo sapiens (Human)
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	IAPP islet amyloid polypeptide [ Homo sapiens ]
Gene Symbol	IAPP
Synonyms	DAP; IAP
Gene ID	3375
Uni Prot ID	P10997
m RNA Refseq	NM_000415.2
Protein Refseq	NP_000406.1
Chromosome Location	12p12.1
Function	hormone activity; receptor binding;
Pathway	Amyloids, organism-specific biosystem; Calcitonin-like ligand receptors, organism-specific biosystem; Class B/2 (Secretin family receptors), organism-specific biosystem; Developmental Biology, organism-specific biosystem; Disease, organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem;
MIM	147940

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Background

Applications

Q & A

Customer Reviews

The Human IAPP Stable Cell Line, using the HeLa cell line, is engineered to express the IAPP (Islet Amyloid Polypeptide) gene. IAPP, also known as amylin, is a hormone co-secreted with insulin by the beta cells of the pancreas. It plays a role in regulating blood sugar levels, but abnormal processing and aggregation of IAPP can lead to the formation of amyloid fibrils, which are associated with type 2 diabetes and other pancreatic disorders. The HeLa cell line, derived from human cervical cancer cells, is widely used in biomedical research for its ability to grow rapidly and support the expression of foreign genes. This stable cell line is important for studying the mechanisms of IAPP aggregation and its implications in disease, as well as for the development of potential therapeutic interventions.

The Human IAPP Stable Cell Line - HeLa is a specialized tool for studying the role of Islet Amyloid Polypeptide (IAPP) in various physiological and pathological conditions. This cell line, which expresses the human IAPP gene, can be utilized in the following applications:

(1) Diabetes Research: IAPP is co-secreted with insulin by pancreatic beta cells and plays a role in regulating blood glucose levels. This cell line can be used to investigate the effects of IAPP on insulin secretion and glucose homeostasis, which is crucial for understanding the pathogenesis of diabetes, particularly type 2 diabetes where IAPP amyloidosis is associated with beta cell dysfunction.

(2) Protein Misfolding and Aggregation Studies: IAPP is prone to misfolding and forming toxic oligomers, which are implicated in the pathogenesis of diabetes and other amyloid-related diseases. The HeLa cell line expressing IAPP can serve as a model to study the molecular mechanisms of IAPP aggregation and to test potential therapeutic agents that prevent or reverse IAPP-induced toxicity.

(3) Drug Development and Screening: The Human IAPP Stable Cell Line can be used for high-throughput screening of compounds that may modulate IAPP expression, secretion, or aggregation. This can lead to the identification of new drugs for the treatment of diabetes and related complications, such as cardiovascular diseases and neuropathy, which are often associated with long-term diabetes.

Customer Q&As

How does the Human IAPP Stable Cell Line - HeLa function in diabetes research?

A: The Human IAPP Stable Cell Line - HeLa, stably expressing human Islet Amyloid Polypeptide (IAPP), serves as an ideal model for studying the relationship between IAPP aggregation and β-cell damage in Type II diabetes. By utilizing this cell line, researchers can evaluate the impact of various factors on IAPP aggregation and its cytotoxic effects, offering new strategies for the prevention and treatment of diabetes.

What impact does overexpression of IAPP have on the apoptosis pathways in the Human IAPP Stable Cell Line - HeLa?

A: In the Human IAPP Stable Cell Line - HeLa, overexpression of IAPP may activate apoptosis pathways. IAPP aggregates can induce oxidative stress, disrupt calcium ion homeostasis, and activate endoplasmic reticulum stress responses, leading to cell apoptosis. Studying these processes helps understand the mechanisms of β-cell dysfunction in diabetes.

Can the Human IAPP Stable Cell Line - HeLa be used to screen compounds that reduce IAPP aggregation?

A: The Human IAPP Stable Cell Line - HeLa provides a unique tool for screening compounds that can reduce IAPP aggregation or mitigate its cytotoxic effects. By evaluating the impact of candidate compounds on IAPP expression, aggregation, and cell viability, potential diabetes treatment drugs can be identified.

How is the Human IAPP Stable Cell Line - HeLa applied in studying the relationship between IAPP and inflammatory responses?

A: The Human IAPP Stable Cell Line - HeLa can be used to study the relationship between IAPP aggregation and inflammatory responses. IAPP aggregates are known to activate innate immune responses, leading to the release of inflammatory factors. This cell line enables exploration of how IAPP influences the development and progression of diabetes through inflammatory pathways.

How does studying IAPP aggregation affect autophagy using the Human IAPP Stable Cell Line - HeLa?

A: The Human IAPP Stable Cell Line - HeLa can be utilized to study how IAPP aggregation affects the autophagy process. IAPP aggregates may interfere with the normal autophagy process, leading to cell dysfunction. This cell line helps reveal the specific interactions between IAPP aggregation and cell autophagy.

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Customer Reviews

Disease Model Construction

The Human IAPP Stable Cell Line - HeLa can be used to construct models of diseases related to Islet Amyloid Polypeptide, providing a foundation for research.

Germany

2021-10-22

Pathological Mechanism Exploration

Through this cell line, researchers can explore the pathological mechanisms of IAPP in diseases such as diabetes.

United States

2022-10-22

Molecular Biology Research

The Human IAPP Stable Cell Line - HeLa supports molecular biology research on IAPP, including gene expression regulation.

Germany

2023-05-11

Protein Aggregation Study

This cell line is suitable for studying the aggregation process of IAPP proteins, which is crucial for understanding amyloidosis diseases.

French

2021-05-19

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Human IAPP Stable Cell Line - HeLa

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Applications

Q & A

Customer Reviews

How does the Human IAPP Stable Cell Line - HeLa function in diabetes research?

What impact does overexpression of IAPP have on the apoptosis pathways in the Human IAPP Stable Cell Line - HeLa?

Can the Human IAPP Stable Cell Line - HeLa be used to screen compounds that reduce IAPP aggregation?

How is the Human IAPP Stable Cell Line - HeLa applied in studying the relationship between IAPP and inflammatory responses?

How does studying IAPP aggregation affect autophagy using the Human IAPP Stable Cell Line - HeLa?

Disease Model Construction

Pathological Mechanism Exploration

Molecular Biology Research

Protein Aggregation Study

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