Cat.No. : CSC-SC007374-1 Host Cell: HeLa
| Cat. No. | CSC-SC007374-1 |
| Description | This cell line is engineered to stably express human islet amyloid polypeptide (IAPP) in HeLa cells. |
| Gene | IAPP |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HeLa |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human IAPP Stable Cell Line - HeLa, stably expressing human Islet Amyloid Polypeptide (IAPP), serves as an ideal model for studying the relationship between IAPP aggregation and β-cell damage in Type II diabetes. By utilizing this cell line, researchers can evaluate the impact of various factors on IAPP aggregation and its cytotoxic effects, offering new strategies for the prevention and treatment of diabetes.
A: In the Human IAPP Stable Cell Line - HeLa, overexpression of IAPP may activate apoptosis pathways. IAPP aggregates can induce oxidative stress, disrupt calcium ion homeostasis, and activate endoplasmic reticulum stress responses, leading to cell apoptosis. Studying these processes helps understand the mechanisms of β-cell dysfunction in diabetes.
A: The Human IAPP Stable Cell Line - HeLa provides a unique tool for screening compounds that can reduce IAPP aggregation or mitigate its cytotoxic effects. By evaluating the impact of candidate compounds on IAPP expression, aggregation, and cell viability, potential diabetes treatment drugs can be identified.
A: The Human IAPP Stable Cell Line - HeLa can be used to study the relationship between IAPP aggregation and inflammatory responses. IAPP aggregates are known to activate innate immune responses, leading to the release of inflammatory factors. This cell line enables exploration of how IAPP influences the development and progression of diabetes through inflammatory pathways.
A: The Human IAPP Stable Cell Line - HeLa can be utilized to study how IAPP aggregation affects the autophagy process. IAPP aggregates may interfere with the normal autophagy process, leading to cell dysfunction. This cell line helps reveal the specific interactions between IAPP aggregation and cell autophagy.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The Human IAPP Stable Cell Line - HeLa can be used to construct models of diseases related to Islet Amyloid Polypeptide, providing a foundation for research.
Through this cell line, researchers can explore the pathological mechanisms of IAPP in diseases such as diabetes.
The Human IAPP Stable Cell Line - HeLa supports molecular biology research on IAPP, including gene expression regulation.
This cell line is suitable for studying the aggregation process of IAPP proteins, which is crucial for understanding amyloidosis diseases.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
