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Human HRAS-G12V Stable Cell Line - Nme

Human HRAS-G12V Stable Cell Line - Nme

Cat.No. :  CSC-RO0679 Host Cell:  Nme

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Cat. No. CSC-RO0679
Description This cell line is engineered to stably overexpress human HRas proto-oncogene, GTPase (HRAS) bearing G12V mutation.
Gene HRAS-G12V
Gene Species Homo sapiens (Human)
Host Cell Nme
Stability Validated for at least 10 passages
Application

1. Gene expression studies

2. Signaling pathway research

3. Drug screening and toxicology

4. Disease research

Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Shipping Dry ice
Storage Liquid nitrogen
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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Easy to use

The operation is very simple. I haven't done anything like this before, but it's easy to practice. I recommend it.

Canada

02/18/2022

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