Human GFRAL Stable Cell Line - HEK293

Name: Human GFRAL Stable Cell Line - HEK293
SKU: CSC-SC006226-1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC006226-1

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC006226-1
Description	This cell line is engineered to stably express human GDNF family receptor alpha like (GFRAL) in HEK293 cells.
Target Gene	GFRAL
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	GFRAL GDNF family receptor alpha like [ Homo sapiens ]
Gene Symbol	GFRAL
Synonyms	GRAL; UNQ9356; C6orf144; bA360D14.1
Gene Description	GDNF family receptor alpha like
Gene ID	389400
Uni Prot ID	Q6UXV0
m RNA Refseq	NM_207410.2
Protein Refseq	NP_997293.2
Chromosome Location	6p12.1

Quick Inquiry

Background

Case Study

Applications

Publications

Q & A

Customer Reviews

GFRAL (Glial cell line-derived neurotrophic factor family receptor alpha-like) is a transmembrane receptor crucial for mediating the metabolic effects of GDF15 (Growth Differentiation Factor 15). Its discovery was elucidated in 2017 by Mullican et al. through a comprehensive bioinformatic analysis of publicly available transcriptomic datasets, pinpointing GFRAL as a hypothalamic gene robustly induced by GDF15. This discovery underscored GFRAL's pivotal role in the central regulation of energy homeostasis.

Concomitantly, the development of a Human GFRAL Stable Cell Line - HEK293 emerged to facilitate further investigation into GFRAL's signaling mechanism and pharmacological modulation. Established through stable transfection of HEK293 cells with GFRAL-expressing constructs, this cell line provides a reliable tool for studying GDF15/GFRAL interactions and downstream signaling pathways. Such a cell model is invaluable for elucidating the physiological relevance and therapeutic potential of targeting the GDF15-GFRAL axis in metabolic regulation.

Growth differentiation factor 15 (GDF15) induces chemotherapy-related nausea, vomiting, and appetite loss by interacting with the GFRAL-RET receptor complex in hindbrain neurons. Researchers have identified GDF15 as a key factor in chemotherapy-induced nausea, emesis, and anorexia by binding to the GFRAL-RET receptor complex in hindbrain neurons. Targeting GDF15-mediated GFRAL-RET signaling shows promise in improving chemotherapy outcomes. Peptide-based antagonists like GRASP block GDF15-mediated RET recruitment. In vivo studies in rats demonstrate that GRASP attenuates GDF15-induced symptoms and synergizes with Ondansetron to further alleviate anorexia. These findings suggest GRASP as a potential treatment for chemotherapy-related malaise and other conditions associated with elevated GDF15 levels.

Figure 1. Flow cytometric analysis of GFRAL–HEK293 cells was conducted by researchers, revealing altered binding patterns in EHMT2 KO cells compared to control cells. HEK293 cells engineered with stable overexpression of human GFRAL were procured from Creative Biogene. (Borner T, et al., 2023)

1. Obesity research: Utilizing Human GFRAL Stable Cell Line - HEK293 to study the effects of GDF15 on energy expenditure and weight regulation in obese animal models.

2. Cancer biology: Investigating the role of GFRAL in cancer cachexia by analyzing the response of Human GFRAL Stable Cell Line - HEK293 to tumor-derived factors.

3. Metabolic disorders: Assessing the therapeutic potential of novel compounds targeting GFRAL signaling using Human GFRAL Stable Cell Line - HEK293 in vitro assays.

4. Drug discovery: Screening small molecule libraries for GFRAL modulators with anti-obesity effects employing Human GFRAL Stable Cell Line - HEK293 as a screening platform.

5. Neurobiology: Examining the neuroprotective properties of GFRAL ligands through Human GFRAL Stable Cell Line - HEK293-based assays in models of neurodegenerative diseases.

Publications

Borner T, Tinsley I C, Milliken B T, et al. Creation of a Peptide Antagonist of the GFRAL–RET Receptor Complex for the Treatment of GDF15-Induced Malaise[J]. Journal of Medicinal Chemistry, 2023.

Customer Q&As

What considerations drove the choice of HEK293 cells for establishing the stable GFRAL cell line?

A: HEK293 cells were likely chosen due to their high transfection efficiency, robust growth characteristics, and suitability for expressing membrane-bound proteins like GFRAL, facilitating studies on its signaling and function.

How was the stability of GFRAL expression assessed and maintained in this HEK293 stable cell line?

A: Stability was likely confirmed through methods such as immunoblotting, flow cytometry, or functional assays assessing downstream signaling pathways, with continuous selection pressure applied.

Can you provide insights into the characterization of GFRAL expression in the HEK293 stable cell line, including its subcellular localization and ligand-binding properties?

A: Characterization may involve analysis of GFRAL localization, ligand-binding kinetics, downstream signaling cascades, and functional implications in metabolic regulation and energy homeostasis.

What quality control measures were implemented during the development of this stable cell line?

A: Quality control likely included screening for mycoplasma contamination, confirmation of stable transgene integration, and assessment of phenotypic stability and consistency.

How does the expression pattern and functional properties of GFRAL in this stable cell line relate to its physiological roles and relevance in metabolic regulation, appetite control, and potential therapeutic targeting?

A: Comparative analysis with in vivo models or clinical data helps validate the relevance of GFRAL expression in metabolic diseases such as obesity, diabetes, and cancer cachexia, guiding therapeutic research efforts.

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Customer Reviews

Eased GDF15 signaling study

This Human GFRAL Stable Cell Line is a lifesaver! Its stable expression in HEK293 cells has made studying GDF15 signaling a lot less daunting.

Germany

2023-08-25

Lifesaver GFRAL expression

Using this cell line feels like having a guiding light! The stable GFRAL expression has clarified my research on metabolic regulation pathways and made my work more impactful.

Germany

2020-06-02

Reliable GFRAL expression

So grateful for this Human GFRAL Stable Cell Line! Its reliable expression in HEK293 cells has brought clarity to my experiments and boosted my confidence in the results.

United Kingdom

2022-04-29

Confidence in results

Can't get over how much smoother my research has become with this cell line! The stable GFRAL expression has simplified my workflow and made studying metabolic regulation mechanisms a joy.

United Kingdom

2024-03-06

Smooth workflow with GFRAL

This Human GFRAL Stable Cell Line is a game-changer! Its consistent expression in HEK293 cells has unlocked new insights into metabolic regulation, making my research journey more rewarding.

Germany

2023-07-23

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Human GFRAL Stable Cell Line - HEK293

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Case Study

Applications

Publications

Q & A

Customer Reviews

Publications

What considerations drove the choice of HEK293 cells for establishing the stable GFRAL cell line?

How was the stability of GFRAL expression assessed and maintained in this HEK293 stable cell line?

Can you provide insights into the characterization of GFRAL expression in the HEK293 stable cell line, including its subcellular localization and ligand-binding properties?

What quality control measures were implemented during the development of this stable cell line?

How does the expression pattern and functional properties of GFRAL in this stable cell line relate to its physiological roles and relevance in metabolic regulation, appetite control, and potential therapeutic targeting?

Eased GDF15 signaling study

Lifesaver GFRAL expression

Reliable GFRAL expression

Confidence in results

Smooth workflow with GFRAL

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