Human FCGR2A(131R)/NFAT-Luc Stable Cell Line - Jurkat

Name: Human FCGR2A(131R)/NFAT-Luc Stable Cell Line - Jurkat
SKU: CSC-RO01141
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO01141

Host Cell : Jurkat Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO01141
Description	This cell line is engineered to constitutively overexpress human FCGR2A(131R), and luciferase reporter gene under the control of NFAT response element. This cell line can be used to evaluate ADCP (antibody-dependent cellular phagocytosis) activity of antibodies in the presence of corresponding target cells.
Target Gene	FCGR2A(131R)/NFAT-Luc
Gene Species	Homo sapiens (Human)
Host Cell	Jurkat
Host Cell Species	Homo sapiens (Human)
Applications	1. Development and evaluation of monoclonal antibodies (mAbs) 2. Development of antibody-based immunotherapies 3. Vaccine development 4. Infectious disease treatment
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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