Human CD52 Stable Cell Line - HEK293

Name: Human CD52 Stable Cell Line - HEK293
SKU: CSC-SC002757-H1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC002757-H1

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC002757-H1
Description	This cell line is engineered to stably overexpress human CD52 molecule (CD52).
Target Gene	CD52
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD52 CD52 molecule [ Homo sapiens ]
Gene Symbol	CD52
Synonyms	CDW52
Gene Description	CD52 molecule
Gene ID	1043
Uni Prot ID	P31358
m RNA Refseq	NM_001803.2
Protein Refseq	NP_001794.2
Chromosome Location	1p36
MIM	114280

Quick Inquiry

Background

Applications

Q & A

Customer Reviews

The Human CD52 gene encodes a cell surface glycoprotein that is involved in cell-cell interactions and is expressed on various cell types, including T cells and some tumor cells. CD52 has been identified as a potential target for cancer immunotherapy, particularly in the context of T cell-based therapies.

The HEK293 cell line, derived from human embryonic kidney, is a versatile model for studying gene expression and protein function. When engineered to stably express the human CD52 gene, this cell line serves as a platform for investigating the role of CD52 in cell biology and its potential as a therapeutic target in cancer. It is also useful for evaluating the efficacy of antibodies or other agents that target CD52 for cancer treatment.

The Human CD52 Stable Cell Line - HEK293 is a cell line that expresses the CD52 antigen, a glycoprotein commonly found on the surface of hematopoietic cells. This cell line is useful for studying the role of CD52 in various biological processes and for the development of therapeutic strategies targeting this protein.

（1）Cancer Research:

CD52 is overexpressed in certain types of cancer, including leukemia and lymphoma. This cell line can be used to investigate the role of CD52 in cancer cell growth, survival, and drug resistance. It can also be employed to test the efficacy of monoclonal antibodies and other drugs targeting CD52 for cancer therapy.

（2）Immunological Studies:

CD52 is involved in T cell activation and regulation. The HEK293 CD52 Stable Cell Line can be utilized to study the interactions between CD52 and other immune system components, contributing to the understanding of T cell-mediated immune responses.

（3）Drug Screening and Development:

The cell line can be used in high-throughput screening assays to identify compounds that modulate CD52 expression or function. This can lead to the discovery of new drugs for treating diseases where CD52 plays a significant role.

Customer Q&As

What is the significance of using the Human CD52 Stable Cell Line - HEK293 for the development of CAR-T cell therapies targeting CD52-expressing malignancies?

A: The Human CD52 Stable Cell Line - HEK293 is an invaluable tool for the development of chimeric antigen receptor (CAR) T cell therapies. It allows researchers to test the specificity and efficacy of CAR-T cells designed to target CD52-expressing cancer cells. By evaluating the cytotoxic activity of CAR-T cells against this cell line, researchers can optimize the design of CAR-T therapies and predict their potential effectiveness in clinical settings.

How can researchers leverage the Human CD52 Stable Cell Line - HEK293 to understand the biodistribution and pharmacokinetics of CD52-targeting therapeutics?

A: Utilizing the Human CD52 Stable Cell Line - HEK293 in animal models enables researchers to study the biodistribution and pharmacokinetics of CD52-targeting therapeutics. By introducing labeled versions of these therapeutics and tracking their accumulation in tissues expressing the CD52 antigen, researchers can gain insights into the drug's distribution, clearance, and potential off-target effects.

What are the experimental strategies that can be employed to assess the impact of CD52 expression levels on the sensitivity of the Human CD52 Stable Cell Line - HEK293 to immunotherapies?

A: Researchers can manipulate the expression levels of CD52 in the Human CD52 Stable Cell Line - HEK293 to investigate how these levels affect the cell line's sensitivity to immunotherapies. This can be achieved through the use of shRNA knockdown, CRISPR/Cas9 gene editing, or overexpression techniques. By comparing the responses of cells with varying CD52 expression levels, researchers can determine the optimal therapeutic window and the potential impact of antigen expression variability on treatment outcomes.

How can the Human CD52 Stable Cell Line - HEK293 be applied to investigate the role of the CD52 antigen in immune cell interactions and signaling processes?

A: By using the Human CD52 Stable Cell Line - HEK293, researchers can explore the functional role of the CD52 antigen in immune cell interactions. This cell line can be co-cultured with immune cells expressing CD52-binding molecules to study the effects on cell signaling, activation, and immune response modulation. Such studies can provide insights into the physiological and pathological roles of CD52 in the immune system.

What are the key characteristics of the Human CD52 Stable Cell Line - HEK293 that make it suitable for studying antibody-drug conjugate (ADC) efficacy and mechanism of action?

A: The Human CD52 Stable Cell Line - HEK293 has been engineered to stably express the human CD52 antigen on its cell surface. This feature is particularly beneficial for researchers interested in evaluating the binding and internalization of ADCs, as CD52 is a common target for such therapeutics. The cell line provides a controlled environment to assess the specificity and cytotoxicity of ADCs, enabling the study of the drug's mechanism of action at a cellular level and informing the design of more effective targeted therapies.

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Customer Reviews

Consistent CD52 Expression

The Human CD52 Stable Cell Line - HEK293 consistently expresses CD52, which is crucial for reliable experimental outcomes in studies focused on this glycoprotein. This consistency ensures reproducible results, which are essential for validating the role of CD52 in immune regulation and potential therapeutic applications.

United Kingdom

2021-06-26

Enhanced Cell Viability

The HEK293 cells are known for their robust growth and high viability, characteristics that extend to the Human CD52 Stable Cell Line - HEK293. This enhanced cell viability is critical for conducting prolonged studies without the risk of losing cell integrity or function.

French

2022-07-07

Useful for Comparative Studies

We can use the Human CD52 Stable Cell Line - HEK293 to perform comparative studies between different cell lines expressing CD52 or between cells with and without CD52 expression. This comparison is vital for understanding the unique roles and effects of CD52 in various cellular environments.

Canada

2024-03-01

Ready for High-Throughput Screening

The Human CD52 Stable Cell Line - HEK293 is particularly suited for high-throughput screening applications. This capability allows for the rapid testing of multiple compounds or conditions affecting CD52 function, streamlining the discovery process in drug development and mechanistic studies of CD52.

United States

2022-08-13

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Human CD52 Stable Cell Line - HEK293

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Applications

Q & A

Customer Reviews

What is the significance of using the Human CD52 Stable Cell Line - HEK293 for the development of CAR-T cell therapies targeting CD52-expressing malignancies?

How can researchers leverage the Human CD52 Stable Cell Line - HEK293 to understand the biodistribution and pharmacokinetics of CD52-targeting therapeutics?

What are the experimental strategies that can be employed to assess the impact of CD52 expression levels on the sensitivity of the Human CD52 Stable Cell Line - HEK293 to immunotherapies?

How can the Human CD52 Stable Cell Line - HEK293 be applied to investigate the role of the CD52 antigen in immune cell interactions and signaling processes?

What are the key characteristics of the Human CD52 Stable Cell Line - HEK293 that make it suitable for studying antibody-drug conjugate (ADC) efficacy and mechanism of action?

Consistent CD52 Expression

Enhanced Cell Viability

Useful for Comparative Studies

Ready for High-Throughput Screening

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