Cat.No. : CSC-RO0680 Host Cell: MDCK-II
| Cat. No. | CSC-RO0680 |
| Description | This cell line is engineered to stably overexpress human ATP binding cassette subfamily B member 1 (ABCB1, also known as CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170; p-170). |
| Gene | ABCB1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | MDCK-II |
| Host Cell Species | Canis lupus familiaris (Dog) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The blood-brain barrier (BBB) is a vital interface that regulates the admission of molecules into the central nervous system, therefore affecting drug distribution in brain tumors and other neurological illnesses. Researchers have looked at the expression and function of transporter proteins at the BBB and found that in glioblastoma microvessels, levels of important transporters such as ABCB1 and ABCG2 were much lower relative to normal brain tissue. This decrease in drug permeability may influence medication delivery to the brain and calls for sensible plans to improve it. The study also utilized MDCKII cell lines, including those stably expressing ABCB1, to evaluate the functional role of these transporters in drug absorption and transport mechanisms.
Figure 1. The researchers assessed the functional impact of ABCB1 and OATP1A2 on fexofenadine transport using MDCKII cell monolayers, measuring intracellular accumulation and apical-to-basolateral transport dynamics. (Bao X, et al., 2020)
Creative Biogene's Human ABCB1 Stable Cell Line - MDCKII is designed to facilitate studies on drug transport mechanisms across the BBB. With its stable expression of ABCB1, this cell line allows researchers to model and investigate the effects of transporter proteins on drug permeability and accumulation.
A: The ABCB1 gene encodes for the P-glycoprotein 1 (P-gp), which is a membrane transporter protein. P-gp is expressed in various tissues, particularly in the intestines, blood-brain barrier, and placenta. It plays a significant role in protecting the body by pumping out foreign substances, toxins, and xenobiotics, including many anticancer drugs.
A: P-glycoprotein functions as an efflux pump, actively transporting a wide range of substrates across cellular membranes against their concentration gradients. This process contributes to the elimination of drugs and other xenobiotics from the body, as well as to the protection of vital organs by limiting the entry of potentially harmful substances.
A: The ABCB1 gene can significantly impact cancer treatment due to its role in drug resistance. Overexpression of P-gp in cancer cells can lead to multidrug resistance, as the protein pumps out anticancer drugs before they can exert their therapeutic effects. This mechanism can reduce the efficacy of chemotherapy and necessitate the use of alternative treatment strategies.
A: Yes, polymorphisms in the ABCB1 gene can influence an individual's response to various medications. Certain variants of the gene may result in altered P-gp function, leading to reduced drug efficacy or increased toxicity. This genetic variability can affect the outcome of treatments for various conditions, including cancer, epilepsy, and HIV/AIDS.
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The Human ABCB1 Stable Cell Line - MDCKII has received positive feedback from the scientific community, with numerous publications in reputable journals showcasing its effectiveness in studying the functions and regulatory mechanisms of the ABCB1 gene. This peer-reviewed recognition highlights the cell line's utility and reliability as a valuable tool for researchers in the field.
The supplier of the Human ABCB1 Stable Cell Line - MDCKII offers exceptional after-sales service, providing prompt assistance and support to address any queries or concerns. Their commitment to customer satisfaction ensures a smooth research experience and fosters confidence in the product.
The Human ABCB1 Stable Cell Line - MDCKII has been widely cited in scientific literature, indicating its significant contribution to the research community. Its recognition in numerous publications highlights its utility and reliability as a valuable tool for studying the functions and regulatory mechanisms of the ABCB1 gene.
The supplier offers a range of stable cell lines, including the Human ABCB1 Stable Cell Line - MDCKII, catering to diverse research needs. This product diversity allows researchers to select the most suitable model for their specific study objectives, enhancing the versatility and applicability of the cell line in various research areas.
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