CDC20B/MIR449C Human Genotyping Kit

Name: CDC20B/MIR449C Human Genotyping Kit
SKU: GK-0221
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : GK-0221

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Product Information

Cat. No.	GK-0221
Description	Leveraging two major technology platforms—KASP (Kompetitive Allele-Specific PCR) and TaqMan MGB probes—we provide cost-effective or ultra-high-specificity custom genotyping services for any species and any genetic locus.
Introduction	If the customer provides a specific genotyping RS number, we can perform custom development and offer SNP testing services upon receipt of mailed blood/tissue/cell samples. The customer needs to provide: 1. Sample materials such as cells (≥10^6 cells), tissue (≥300 mg), blood (≥1 ml), serum (≥1.5 ml), etc.; nucleic acid extraction is charged separately; 2. Genomic DNA (volume ≥30 μl, concentration ≥50 ng/μl), with purity OD260/280 between 1.7 and 1.9; 3. For the human genome, the RS number of the SNP locus must be provided. For other species without rs numbers, such as cattle, chickens, and fish, the exact 200 bp sequences flanking both upstream and downstream of the SNP locus must be provided, along with the mutation type at the SNP locus and whether any other linked loci exist within 25 bp upstream or downstream of the locus. We deliver: 1. SNP results (Excel spreadsheet); 2. Complete experimental report: amplification and reaction systems, primers involved, and probe sequences; Scatter plot of clustering results (KASP method) / Amplification curves and Ct value analysis (TaqMan MGB method). 3. Other relevant materials required by the customer.
Gene	CDC20B/MIR449C
Species	Human
Features	Genotyping Accuracy >99%
Applications	Research Use
Size	Box
Format	100T
Method	Competitive Allele-Specific Targeting PCR (CastPCR) is a high-throughput, low-cost genotyping technology based on competitive allele-specific PCR for SNP (single nucleotide polymorphism) and InDel (insertion/deletion) detection, widely used in fields such as plant and animal breeding and medical genetic testing. Its principle is the same as that of PCR Allele Competitive Extension (PACE), Allele-Specific Quantitative PCR based genotyping assay (AQP), Amplification with fluorescence (Amplifluor), Penta-primer Amplification Refractory Mutation System (PARMS), Kompetitive Allele-Specific PCR (KASP), and Competitive Allele-Specific PCR (CASP).
Note	All SNP kits are custom-developed and not available from stock. Primers and probes are individually designed and synthesized based on the RS numbers or target sequences provided by the customer.
Storage	-20℃

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