AAV5-CAG-NLuc-2A-GFP

AAV5-CAG-NLuc-2A-GFP is a recombinant adeno-associated virus (AAV) serotype 5 vector engineered for efficient dual reporter gene expression. This vector utilizes the CAG promoter (a hybrid of the CMV enhancer and chicken β-actin promoter) to ensure robust and widespread expression in various cell types. Its unique design incorporates a 2A self-cleaving peptide, enabling co-expression of NanoLuc luciferase (NLuc) and green fluorescent protein (GFP) from the same transcript, allowing for simultaneous quantitative (luminescence) and qualitative (fluorescence) tracking of transduction efficiency. The AAV5 capsid protein enhances its tropism for central nervous system (CNS) target cells, including neurons and glial cells, while minimizing immunogenicity—a crucial feature for AAV safety. Combining high-titer production, stable long-term expression, and dual reporter genes, this vector is ideal for sensitive, long-term studies.

The applications of AAV5-CAG-NLuc-2A-GFP span basic and preclinical research. In gene therapy development, it serves as a tracer to monitor vector biodistribution and transduction kinetics through non-invasive bioluminescence imaging (BLI) and fluorescence microscopy. Researchers utilize the NLuc reporter gene, which is over 100 times brighter than firefly luciferase, to track the expression of low-abundance genes in vivo, while GFP aids in cell-type-specific validation. This vector is particularly valuable in fields such as neuroscience (e.g., mapping neural circuits), oncology (tumor model labeling), and vaccine immunology (antigen presentation studies).