Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0084
Target Gene : Zdhhc9 Host Cell : NIH3T3
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0084 |
| Description | NIH3T3-Zdhhc9 cell line is a stable cell line with a homozygous knockout of Zdhhc9 |
| Target Gene | Zdhhc9 |
| Host Cell | NIH3T3 |
| Host Cell Species | Mus musculus (Mouse) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Target Gene | ZDHHC9 |
| Background | This gene encodes an integral membrane protein that is a member of the zinc finger DHHC domain-containing protein family. The encoded protein forms a complex with golgin subfamily A member 7 and functions as a palmitoyltransferase. This protein specifically palmitoylates HRAS and NRAS. Mutations in this gene are associated with X-linked cognitive disability. Alternate splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, May 2010] |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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The clear instructions and user-friendly protocol of TZdhhc9 Knockout Cell Line-NIH3T3 made it easy for me to get started with gene editing.
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Zdhhc9 Knockout Cell Line-NIH3T3 has made gene editing a breeze. I am amazed by how quickly and efficiently it works.
I have used Zdhhc9 Knockout Cell Line-NIH3T3 for several experiments now, and it has consistently produced excellent results. I highly recommend it to any user.
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